CD55 Antikörper

Produktnummer ABIN5541172

Dieses Anti-CD55-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von CD55 in WB, FACS, IHC (fro), Func und EIA. Geeignet für Ratte.

Kurzübersicht für CD55 Antikörper (ABIN5541172)

Target: CD55 (Complement Decay-Accelerating Factor (CD55))

Reaktivität: Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD55 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func), Enzyme Immunoassay (EIA)

Klon: RDIII-24

Produktdetails anti-CD55 Antikörper

Spezifität: The monoclonal antibody RDII-24 recognizes complement decay accelerating factor (DAF), also designated as CD55.

Aufreinigung: Purified

Immunogen: NIH-3T3 cells expressing GPI-anchored Rat DAF

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow Cytometry (Ref.1,2). Western blot: Non-reduced with a band size of 60-70 kDa. As positive control CHO-Rat DAF hyper-expressing cells were used (Ref.1,2). Functional Studies: RDIII-7 antibody completely blocked the protective effect of expressed Rat DAF (Ref.2). Immuno Assays (Ref.3). Immunohistochemistry on Frozen Sections (Ref.2). The typical starting working dilution is 1/50.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Buffer System: PBS Stabilizers: 0.1 % BSA

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 2-8°C. DO NOT FREEZE! Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu CD55

Target: CD55 (Complement Decay-Accelerating Factor (CD55))

Andere Bezeichnung: cd55,daf

Hintergrund: Cells express on their surface several proteins which protect against complement attack, namely C receptor I (CR1), decay accelerating factor (DAF), membrane cofactor protein (MCP) and CD59. CR1, DAF and MCP regulate the activation pathways of complement by either accelerating decay of the C3 and C5 convertase (CR1, DAF), or acting as cofactors for the serine protease factor I, which cleaves and irreversibly inactivates C3b (CR1, MCP). Rat DAF (CD55) is a 60 kDa transmembrane protein that binds C3b and C4b to inhibit formation and half-life of the C3 convertases. It belongs to the receptors of complement activation (RCA) family. DAF is broadly distributed among cells in contact with plasma complment proteins, including both haematopoietic and nonhaematopoietic cells. Although DAF does not have an essential role in controlling hemolysis of erythrocytes, it has an important role in regulation of the deposition of C3 on nucleated cells. Together with other complement regulators DAF protects self cells from autologous complement-mediated injury. DAF cooperates with CD46 in circumventing autologous C3 deposition, while CD59 inhibits the pathway at the critical end-point.

UniProt: Q9QYJ9

Pathways: Komplementsystem, Regulation of Leukocyte Mediated Immunity