beta Actin Antikörper (N-Term)

Produktnummer ABIN5540759

Dieses Maus Monoklonal-Antikörper erkennt spezifisch beta Actin in WB, IF, IHC (p), ICC, FACS, IHC (fro) und EIA. Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte, Huhn, Kaninchen, Schwein und Zebrafisch (Danio rerio).

Kurzübersicht für beta Actin Antikörper (N-Term) (ABIN5540759)

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Reaktivität: Human, Maus, Ratte, Huhn, Kaninchen, Schwein, Zebrafisch (Danio rerio)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser beta Actin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Klon: 4C2

Produktdetails anti-beta Actin Antikörper

Bindungsspezifität: N-Term

Sequenz: Ac-DDDIAALVV-COOH

Spezifität: This 4C2 Monoclonal antibody is highly specific for β-Cytoplasmic Actin. The antibody does not cross react with other Actin isoforms.

Aufreinigung: Purified

Immunogen: Peptide comprising the N-terminal nonapeptide of beta-Cytoplasmic Actin with an acetylated N-terminus coupled to KLH through the cysteine residue

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: ELISA. Flow cytometry. Western blot. Immunocytochemistry. Immunohistochemistry on Frozen sections. Immunohistochemistry on Paraffin-embedded sections. Recommended Dilutions : 1/50-1/100 for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent and 1/100-1/500 for immunoblotting applications.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Buffer System: PBS Preservatives: 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freeze-thaw cycles. Shelf life: One year from despatch.

Details zu beta Actin

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Andere Bezeichnung: actin beta,actb

Hintergrund: Among the six actin isoforms described in mammals, two are found in virtually all cells (β- and γ-cytoplasmic), two are detected in smooth muscle cells (α- and γ-smooth muscle) and two are present in striated muscles, one predominantly in skeletal (α-skeletal) and one in cardiac (α-cardiac) muscle cells. These actin isoforms differ slightly in their N-terminus, but the sequence of each of these actins is highly conserved in higher vertebrates. β- and γ-cytoplasmic actin play crucial roles during various key cellular processes. Whereas β-actin is preferentially localized in stress fibers, circular bundles and at cell-cell contacts, suggesting a role in cell attachment and contraction, γ-actin displays a more versatile organization, according to cell activities. In moving cells, γ-actin is mainly organized as a meshwork in cortical and lamellipodial structures, suggesting a role in cell motility. β- and γ-actin depleted fibroblasts exhibit distinct changes in motility compared with their controls, suggesting a specific role for each isoform in cell locomotion.

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction