CD51 Antikörper

Produktnummer ABIN5540182

Dieses Anti-CD51-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von CD51 in FACS, IP, IHC (fro) und EIA. Geeignet für Human.

Kurzübersicht für CD51 Antikörper (ABIN5540182)

Target: CD51 (ITGAV) (Integrin alpha V (ITGAV))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD51 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Klon: NKI-M7 (former AMF7)

Produktdetails anti-CD51 Antikörper

Spezifität: T-lymphocyte clones NKI-M7 detect a melanoma-associated proteoglycan (MW greater than 450-250 kDa) and a molecular complex, which under reducing conditions consists of 4 compounds of 120, 95, 29 and 25 kDa respectively. reacts with cultured melanomas melanomas in frozen sections. The antigen detected by is strongly associated with the adhesion and cytoplasmic spreading of melanoma cells to plastic surfaces and monolayers of vascular endothelial cells. blocks Fibronectin-induced chemotaxic motility and chemokinesis of melanoma cells. In addition to their membrane localization, the antigens detected by NKI-M7 are abundant in extracellular adhesion plaques deposited by cultured melanoma cells. stains primary tumors heterogeneously, whereas metastases are homogeneously stained.

Aufreinigung: Purified

Immunogen: Derived by fusion of SP2/0 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with cultured melanoma cells derived from a Human melanoma metastasis.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: ELISA. Fow Cytometry. Immunoprecipitation. Immunohistochemistry on Frozen Sections.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Buffer System: PBS Preservatives: 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu CD51

Target: CD51 (ITGAV) (Integrin alpha V (ITGAV))

Andere Bezeichnung: cd51,itgav

Hintergrund: CD51 (Cluster of Differentiation 51), known as vitronectin receptor α chain, or integrin αV is a type I integral membrane glycoprotein. CD51 forms a heterodimer with integrin β1 (CD29), β3 (CD61), β5, β6, or β8. CD51 contains two disulfide-linked subunits of 125 kD and 24 kD. Many extracellular matrix proteins with RGD-motifs are CD51 ligands. In association with its β chains, CD51 binds vitronectin, von Willebrand factor, fibronectin, thrombospondin, osteopontin, fibrinogen, and laminin. CD51, as an adhesion molecule, plays important roles in leukocytes homing and rolling, mediates bone absorption and angiogenesis. CD51 is expressed on endothelial cells, fibroblasts, macrophages, platelets, osteoclasts, neuroblastoma, melanoma, and hepatoma cells.

UniProt: P06756

Pathways: Cell-Cell Junction Organization, Signaling Events mediated by VEGFR1 and VEGFR2, Growth Factor Binding, Integrin Complex