NSG2 Antikörper (C-Term)

Produktnummer ABIN5539930

Produktdetails anti-NSG2 Antikörper

Target: NSG2 (HMP19) (Neuron-Specific Protein Family Member 2 (HMP19))

Bindungsspezifität: C-Term

Reaktivität: Maus, Ratte

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser NSG2 Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Immunocytochemistry (ICC)

Verwendungszweck: Nsg2 (mouse, C terminus)

Sequenz: HEPKPPKTQG H

Spezifität: Reported variants represent identical protein: NP_001277610.1, NP_001277609.1, NP_032767.1

Kreuzreaktivität: Maus

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Güteklasse: Verified

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx 19 kDa band observed in Mouse fetal Brain lysates (calculated MW of 19.0 kDa according to NP_032767.1). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation was 1 hour. This antibody has been successfully used in WB on Rat: Chan

Peptide ELISA: antibody detection limit dilution 1:128000.

Kommentare:

Immunocytochemistry: This antibody has been successfully used in ICC on Rat: Chan Choo Yap et al. (2022) PMID: 35474277.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Referenzen für anti-NSG2 Antikörper (ABIN5539930)

Yap, Digilio, McMahon, Garcia, Winckler: "Degradation of dendritic cargos requires Rab7-dependent transport to somatic lysosomes." in: The Journal of cell biology, Vol. 217, Issue 9, pp. 3141-3159, (2019) (PubMed).

Yap, Digilio, McMahon, Winckler: "The endosomal neuronal proteins Nsg1/NEEP21 and Nsg2/P19 are itinerant, not resident proteins of dendritic endosomes." in: Scientific reports, Vol. 7, Issue 1, pp. 10481, (2019) (PubMed).

Details zu NSG2

Target: NSG2 (HMP19) (Neuron-Specific Protein Family Member 2 (HMP19))

Andere Bezeichnung: Nsg2 (HMP19 Produkte)

Synonyme: NSG2 antikoerper, 8.5 antikoerper, AA989750 antikoerper, R75287 antikoerper, neuronal vesicle trafficking associated 2 antikoerper, neuron specific gene family member 2 antikoerper, NSG2 antikoerper, Nsg2 antikoerper

Hintergrund: Nsg2, neuron specific gene family member 2, protein 8.5, protein p19

Gen-ID: 18197

NCBI Accession: NP_032767