CD3 epsilon Antikörper (Internal Region)
Kurzübersicht für CD3 epsilon Antikörper (Internal Region) (ABIN5539697)
Target
Alle CD3 epsilon (CD3E) Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
Güteklasse
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Bindungsspezifität
- Internal Region
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Verwendungszweck
- CD3E (cytoplasmic)
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Sequenz
- RKGQRDLYSG LNQR
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Spezifität
- This antibody is designed to recognize the cytoplasmic domain (aa153-207) of the protein.
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Kreuzreaktivität
- Human, Schwein, Ratte
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Aufreinigung
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Isotyp
- IgG
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anti-CD3 epsilon (CD3E) (AA 157-207) antibody
CD3E Reaktivität: Human FACS, WB, IHC, IP, IF (p), mIF Wirt: Kaninchen Monoclonal BL-298-5D12 unconjugated Recombinant Antibody
anti-CD3 epsilon (CD3E) (AA 23-207) antibodyCD3E Reaktivität: Human, Maus, Ratte, Huhn WB, IHC, IF, ICC, IHC (fro) Wirt: Kaninchen Polyclonal unconjugated
anti-CD3 epsilon (CD3E) (C-Term) antibodyCD3E Reaktivität: Human, Maus, Ratte WB, IHC, IF, ELISA, ICC Wirt: Kaninchen Polyclonal unconjugated
anti-CD3 epsilon (CD3E) (AA 156-168), (Cytoplasmic Domain) antibodyCD3E Reaktivität: Human, Ratte, Schwein, Rind (Kuh), Pferd, Kaninchen, Meerschweinchen, Huhn FACS, WB, IF Wirt: Maus Monoclonal PC3-188A unconjugated
anti-CD3 epsilon (CD3E) (AA 23-119) antibodyCD3E Reaktivität: Human FACS, WB, IHC, ELISA Wirt: Maus Monoclonal C3e-1931 unconjugated
anti-CD3 epsilon (CD3E) antibodyCD3E Reaktivität: Human, Maus, Ratte FACS, IF, Func Wirt: Maus Monoclonal RIV9 unconjugated
anti-CD3 epsilon (CD3E) (AA 25-207) antibodyCD3E Reaktivität: Human WB, IHC, IP Wirt: Kaninchen Polyclonal unconjugated
anti-CD3 epsilon (CD3E) (C-Term) antibodyCD3E Reaktivität: Human WB, IHC, IF Wirt: Kaninchen Polyclonal unconjugated
anti-CD3 epsilon (CD3E) antibodyCD3E Reaktivität: Human, Maus, Ratte WB, IHC, IF Wirt: Kaninchen Polyclonal unconjugated
anti-CD3 epsilon (CD3E) antibodyCD3E Reaktivität: Human WB, IHC, IF Wirt: Kaninchen Chimeric YTH 12-5 unconjugated Recombinant Antibody
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Applikationshinweise
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Western Blot: Approx 22 kDa band observed in lysates of cell lines Jurkat and Molt4 (calculated MW of 23.1 kDa according to NP_000724.1). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation was 1 hour. This antibody has been successfully used in WB
Peptide ELISA: antibody detection limit dilution 1:128000.
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Kommentare
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Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konzentration
- 0.5 mg/mL
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Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handhabung
- Minimize freezing and thawing.
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Lagerung
- -20 °C
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Informationen zur Lagerung
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- CD3 epsilon (CD3E)
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Andere Bezeichnung
- CD3E
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Hintergrund
- CD3E, CD3e molecule, epsilon (CD3-TCR complex), IMD18, T3E, TCRE, CD3-epsilon, CD3e antigen, epsilon polypeptide (TiT3 complex), T-cell antigen receptor complex, epsilon subunit of T3, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycopro
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Gen-ID
- 916, 315609
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NCBI Accession
- NP_000724
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Pathways
- T-Zell Rezeptor Signalweg, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway
Target
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