CD3 epsilon Antikörper (Internal Region)

Produktnummer ABIN5539697

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch CD3 epsilon in WB und ELISA. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für CD3 epsilon Antikörper (Internal Region) (ABIN5539697)

Target: CD3 epsilon (CD3E)

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser CD3 epsilon Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Güteklasse: Verified

Produktdetails anti-CD3 epsilon Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: CD3E (cytoplasmic)

Sequenz: RKGQRDLYSG LNQR

Spezifität: This antibody is designed to recognize the cytoplasmic domain (aa153-207) of the protein.

Kreuzreaktivität: Human, Schwein, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx 22 kDa band observed in lysates of cell lines Jurkat and Molt4 (calculated MW of 23.1 kDa according to NP_000724.1). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation was 1 hour. This antibody has been successfully used in WB

Peptide ELISA: antibody detection limit dilution 1:128000.

Kommentare:

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu CD3 epsilon

Target: CD3 epsilon (CD3E)

Andere Bezeichnung: CD3E

Hintergrund: CD3E, CD3e molecule, epsilon (CD3-TCR complex), IMD18, T3E, TCRE, CD3-epsilon, CD3e antigen, epsilon polypeptide (TiT3 complex), T-cell antigen receptor complex, epsilon subunit of T3, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycopro

Gen-ID: 916, 315609

NCBI Accession: NP_000724

Pathways: T-Zell Rezeptor Signalweg, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway