UCP2 Antikörper (Internal Region)

Produktnummer ABIN5539644

Der Ziege Polyklonal Anti-UCP2-Antikörper wurde für WB, ELISA und IF validiert. Er ist geeignet, UCP2 in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für UCP2 Antikörper (Internal Region) (ABIN5539644)

Target: UCP2 (Uncoupling Protein 2 (Mitochondrial, Proton Carrier) (UCP2))

Reaktivität: Human, Maus, Ratte

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser UCP2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Güteklasse: Verified

Produktdetails anti-UCP2 Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: Uncoupling protein 2 / UCP2

Sequenz: DSVKQFYTKG SEH

Kreuzreaktivität: Hund, Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: Weak positive staining of red and white pulp cells in parafin embedded Human Kidney, at 6-7 μg/mL.

Western Blot: Approx 37 kDa band observed in Rat Adipose and 28-30 kDa in Mouse Spleen lysates (calculated MW of 33.4 kDa according to Mouse NP_035801.3 and 33.4 kDa according to Rat NP_062227.2). Recommended concentration: 0.3-1 μg/mL. Primary incubation 1 h

Peptide ELISA: antibody detection limit dilution 1:128000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the Mitochondria of MCF7 cells. Recommended concentration: 10μg/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu UCP2

Target: UCP2 (Uncoupling Protein 2 (Mitochondrial, Proton Carrier) (UCP2))

Andere Bezeichnung: UCP2

Hintergrund: UCP2, uncoupling protein 2 (mitochondrial, proton carrier), SLC25A8, UCPH , uncoupling protein 2, uncoupling protein-2

Gen-ID: 7351, 22228, 54315

NCBI Accession: NP_003346

Pathways: Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Proton Transport