CRTC2 Antikörper (C-Term)

Produktnummer ABIN5539499

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch CRTC2 in WB, ELISA, IF und FACS. Er zeigt eine Reaktivität gegenüber Human und Ratte.

Kurzübersicht für CRTC2 Antikörper (C-Term) (ABIN5539499)

Target: CRTC2 (CREB Regulated Transcription Coactivator 2 (CRTC2))

Reaktivität: Human, Ratte

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser CRTC2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Güteklasse: Verified

Produktdetails anti-CRTC2 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: CRTC2

Sequenz: DPAVEDSFRS DRLQ

Kreuzreaktivität: Rind (Kuh), Hund, Human, Maus, Schwein, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx 90 kDa observed in lysates of cell line Jurkat and in preliminary testing of NIH3T3, and approx 85 kDa in Rat Brain lysates and lysates of cell line A431 (calculated MW of 73.2 kDa according to Human, NP_083157.1) and Rat NP_001029067

Peptide ELISA: antibody detection limit dilution 1:64000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10u

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu CRTC2

Target: CRTC2 (CREB Regulated Transcription Coactivator 2 (CRTC2))

Andere Bezeichnung: CRTC2

Hintergrund: Crtc2, CREB regulated transcription coactivator 2, 4632407F12Rik, Torc2, OTTMUSP00000023505, transducer of regulated cAMP response element-binding protein (CREB) 2

Gen-ID: 200186, 74343, 310615

NCBI Accession: NP_083157

Pathways: AMPK Signaling, Carbohydrate Homeostasis