NSG1 Antikörper (N-Term)

Produktnummer ABIN5539461

Der Ziege Polyklonal Anti-NSG1-Antikörper wurde für WB, ELISA und ICC validiert. Er ist geeignet, NSG1 in Proben von Maus und Ratte zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für NSG1 Antikörper (N-Term) (ABIN5539461)

Target: NSG1 (D4S234E) (Neuron-Specific Protein Family Member 1 (D4S234E))

Reaktivität: Maus, Ratte

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser NSG1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Güteklasse: Verified

Produktdetails anti-NSG1 Antikörper

Bindungsspezifität: N-Term

Verwendungszweck: Nsg1 / Neep21

Sequenz: EKGTKQPLLE DGFD

Kreuzreaktivität: Rind (Kuh), Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx 22 kDa band observed in Mouse fetal Brain lysates (calculated MW of 20.9 kDa according to NP_035072.2). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:128000.

Kommentare:

Immunocytochemistry: Primary DIV9 cells from Hippocampus E18 Rat embryos, stained for neuronal marker MAP2 in blue and for glial marker GFAP in green showed exclusive labelling of EB11637 (red) in the neurons only. Recommended concentrat

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Referenzen für anti-NSG1 Antikörper (ABIN5539461)

Digilio, Yap, Winckler et al.: "Ctip2-, Satb2-, Prox1-, and GAD65-Expressing Neurons in Rat Cultures: Preponderance of Single- and Double-Positive Cells, and Cell Type-Specific Expression of Neuron-Specific Gene Family Members, ..." in: PLoS ONE, Vol. 10, Issue 10, pp. e0140010, (2015) (PubMed).

Details zu NSG1

Target: NSG1 (D4S234E) (Neuron-Specific Protein Family Member 1 (D4S234E))

Andere Bezeichnung: Nsg1

Hintergrund: Nsg1, neuron specific gene family member 1, Bsmrb, Neep21, PEP1, brain neuron cytoplasmic protein 1/2, brain specific mRNA b, neuron-specific protein family member 1

Gen-ID: 27065, 18196, 25247

NCBI Accession: NP_077042