SUPT5H Antikörper (AA 395-424)

Produktnummer ABIN5531040

Der Kaninchen Polyklonal Anti-SUPT5H-Antikörper wurde für WB validiert. Er ist geeignet, SUPT5H in Proben von Human zu detektieren.

Kurzübersicht für SUPT5H Antikörper (AA 395-424) (ABIN5531040)

Target: SUPT5H (Transcription Elongation Factor SPT5 (SUPT5H))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SUPT5H Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-SUPT5H Antikörper

Bindungsspezifität: AA 395-424

Aufreinigung: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This SUPT5H antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 395-424 amino acids from the Central region of human SUPT5H.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: For WB starting dilution is: 1:1000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.3 mg/mL

Buffer: Supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Details zu SUPT5H

Target: SUPT5H (Transcription Elongation Factor SPT5 (SUPT5H))

Andere Bezeichnung: SUPT5H

Hintergrund: Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences.

Molekulargewicht: 121 kDa

Gen-ID: 6829

UniProt: O00267