POLI Antikörper (C-Term)

Produktnummer ABIN5531016

Der Kaninchen Polyklonal Anti-POLI-Antikörper wurde für WB und IHC (p) validiert. Er ist geeignet, POLI in Proben von Human zu detektieren.

Kurzübersicht für POLI Antikörper (C-Term) (ABIN5531016)

Target: POLI (Polymerase (DNA Directed) iota (POLI))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser POLI Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-POLI Antikörper

Bindungsspezifität: AA 673-701, C-Term

Aufreinigung: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This POLI antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 673-701 amino acids from the C-terminal region of human POLI.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Details zu POLI

Target: POLI (Polymerase (DNA Directed) iota (POLI))

Andere Bezeichnung: POLI

Hintergrund: Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunogobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity.

Molekulargewicht: 83 kDa

Gen-ID: 11201

UniProt: Q9UNA4

Pathways: DNA Reparatur