MED20 Antikörper (AA 1-212)

Produktnummer ABIN5519037

Produktdetails anti-MED20 Antikörper

Target: MED20 (Mediator Complex Subunit 20 (MED20))

Bindungsspezifität: AA 1-212

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MED20 Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB)

Verwendungszweck: Anti-TRFP/MED20 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-TRFP/MED20 Antibody Picoband® (ABIN5519037). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E. coli-derived human TRFP / MED20 recombinant protein (Position: M1-R212).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL
ELISA, 0.1-0.5 μg/mL
1. Sato, S., Tomomori-Sato, C., Banks, C. A. S., Parmely, T. J., Sorokina, I., Brower, C. S., Conaway, R. C., Conaway, J. W. A mammalian homolog of Drosophila melanogaster transcriptional coactivator Intersex is a subunit of the mammalian Mediator complex. J. Biol. Chem. 278: 49671-49674, 2003. 2. Xiao, H., Tao, Y., Roeder, R. G. The human homologue of Drosophila TRF-proximal protein is associated with an RNA polymerase II-SRB complex. J. Biol. Chem. 274: 3937-3940, 1999.

Kommentare:

We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu MED20

Target: MED20 (Mediator Complex Subunit 20 (MED20))

Andere Bezeichnung: MED20 (MED20 Produkte)

Hintergrund:

Synonyms: Mediator of RNA polymerase II transcription subunit 20, Mediator complex subunit 20, TRF-proximal protein homolog, hTRFP, MED20, TRFP,

Tissue Specificity: Pre-B-cells and B-cells but not terminally differentiated plasma cells.

Background: MED20 is a component of the mediator complex (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. A mutation in this gene has been associated with a novel infantile-onset neurodegenerative movement disorder.

Molekulargewicht: 23 kDa

Gen-ID: 9477

UniProt: Q9H944

Pathways: Regulation of Lipid Metabolism by PPARalpha