Phenylalanine Hydroxylase Antikörper (AA 71-208)

Produktnummer ABIN5518942

Der Kaninchen Polyklonal Anti-Phenylalanine Hydroxylase-Antikörper wurde für WB, IHC und FACS validiert. Er ist geeignet, Phenylalanine Hydroxylase in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für Phenylalanine Hydroxylase Antikörper (AA 71-208) (ABIN5518942)

Target: Phenylalanine Hydroxylase

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Phenylalanine Hydroxylase Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Produktdetails anti-Phenylalanine Hydroxylase Antikörper

Bindungsspezifität: AA 71-208

Verwendungszweck: Anti-PAH Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-PAH Antibody Picoband® (ABIN5518942). Tested in Flow Cytometry(Intracellular), IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E. coli-derived human PAH recombinant protein (Position: R71-H208). Human PAH shares 89.1% and 88.4% amino acid (aa) sequence identity with mouse and rat PAH, respectively.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat,
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Fitzpatrick PF (1999). "Tetrahydropterin-dependent amino acid hydroxylases". Annual Review of Biochemistry. 68: 355-81. 2. Kaufman S (Feb 1958). "A new cofactor required for the enzymatic conversion of phenylalanine to tyrosine". The Journal of Biological Chemistry. 230 (2): 931-9.

Kommentare:

We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu Phenylalanine Hydroxylase

Target: Phenylalanine Hydroxylase

Andere Bezeichnung: PAH

Hintergrund:

Synonyms: Phenylalanine-4-hydroxylase,PAH,1.14.16.1,Phe-4-monooxygenase,PAH,

Tissue Specificity: Predominantly expressed in lung and at lower level in kidney. Expressed in macrophages but not in vascular smooth muscle cells.

Background: Phenylalanine hydroxylase (PAH) is an enzyme that catalyzes the hydroxylation of the aromatic side-chain of phenylalanine to generate tyrosine. It is one of three members of the biopterin-dependent aromatic amino acid hydroxylases, a class of monooxygenase that uses tetrahydrobiopterin (BH4, a pteridine cofactor) and a non-heme iron for catalysis. Deficiency of this enzyme activity results in the autosomal recessive disorder phenylketonuria.

Molekulargewicht: 45 kDa

Gen-ID: 5053

UniProt: P00439