ATG3 Antikörper (AA 1-244)

Produktnummer ABIN5518892

Dieses Anti-ATG3-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von ATG3 in WB. Geeignet für Human, Maus und Affe.

Kurzübersicht für ATG3 Antikörper (AA 1-244) (ABIN5518892)

Target: ATG3 (ATG3 Autophagy Related 3 (ATG3))

Reaktivität: Human, Maus, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ATG3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-ATG3 Antikörper

Bindungsspezifität: AA 1-244

Verwendungszweck: Anti-Apg3/ATG3 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-Apg3/ATG3 Antibody Picoband® (ABIN5518892). Tested in WB applications. This antibody reacts with Human, Monkey, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human < a href="https://www.bosterbio.com/anti-apg3-atg3-rabbit-monoclonal-antibody-m01768-boster.html" style="color:#EA8D28">Apg3 recombinant protein (Position: M1-T244). Human Apg3 shares 96.7% and 96.3% amino acid (aa) sequence identity with mouse and rat Apg3, respectively.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human, Monkey, Mouse
1. Tanida, I., Tanida-Miyake, E., Komatsu, M., Ueno, T., Kominami, E. Human Apg3p/Aut1p homologue is an authentic E2 enzyme for multiple substrates, GATE-16, GABARAP, and MAP-LC3, and facilitates the conjugation of hApg12p to hApg5p. J. Biol. Chem. 277: 13739-13744, 2002. 2. Tsuboyama, K., Koyama-Honda, I., Sakamaki, Y., Koike, M., Morishita, H., Mizushima, N. The ATG conjugation systems are important for degradation of the inner autophagosomal membrane. Science 354: 1036-1041, 2016.

Kommentare:

We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu ATG3

Target: ATG3 (ATG3 Autophagy Related 3 (ATG3))

Andere Bezeichnung: ATG3

Hintergrund:

Synonyms: Ubiquitin-like-conjugating enzyme ATG3,6.3.2.-,Autophagy-related protein 3,APG3-like,hApg3,Protein PC3-96,ATG3,APG3, APG3L,

Tissue Specificity: Widely expressed, with a highest expression in heart, skeletal muscle, kidney, liver and placenta. .

Background: This ATG3 gene encodes a ubiquitin-like-conjugating enzyme and is a component of ubiquitination-like systems involved in autophagy, the process of degradation, turnover and recycling of cytoplasmic constituents in eukaryotic cells. And the protein is known to play a role in regulation of autophagy during cell death. A pseudogene of this gene is located on chromosome 20. Alternative splicing results in multiple transcript variants encoding different isoforms.

Molekulargewicht: 36-40 kDa

Gen-ID: 64422

Pathways: Autophagie