Vinculin Antikörper (AA 930-1134)

Produktnummer ABIN5518889

Dieses Anti-Vinculin-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Vinculin in WB. Geeignet für Human, Maus und Ratte. Dieses Primary Antibody wurde in 3+ Publikationen zitiert.

Kurzübersicht für Vinculin Antikörper (AA 930-1134) (ABIN5518889)

Target: Vinculin (VCL)

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Vinculin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-Vinculin Antikörper

Bindungsspezifität: AA 930-1134

Verwendungszweck: Anti-Vinculin/VCL Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-Vinculin/VCL Antibody Picoband® (ABIN5518889). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human Vinculin recombinant protein (Position: E930-Q1134). Human Vinculin shares 87.7% amino acid (aa) sequence identity with both mouse and rat Vinculin.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Ezzell RM, Goldmann WH, Wang N, Parashurama N, Parasharama N, Ingber DE (1997). "Vinculin promotes cell spreading by mechanically coupling integrins to the cytoskeleton". Exp. Cell Res. 231 (1): 14-26. 2. Goldmann WH, Ingber DE (2001). "Intact vinculin protein is Require for control of cell shape, cell mechanics, and rac-Dependent Lamellipodia Formation". Biochemical and Biophysical Research Communications. 290 (2): 749-755. 3. Xu W, Baribault H, Adamson ED (1998). "vinculin knockout results in heart and brain defects during embryonic development". Development. 125(2): 327-337.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.005 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Referenzen für anti-Vinculin Antikörper (ABIN5518889)

Cong, Fu, Zhang, Zhao, Zhang: "Effects of atorvastatin on porcine aqueous humour outflow and trabecular meshwork cells." in: Experimental and therapeutic medicine, Vol. 15, Issue 1, pp. 210-216, (2018) (PubMed).

Shen, Lu, Li, Zhang, Zhu: "Synthesis and characterization of biodegradable polyurethane for hypopharyngeal tissue engineering." in: BioMed research international, Vol. 2015, pp. 871202, (2015) (PubMed).

Su, Liu, Ma, Wang, E, Liu: "Enhancement of periodontal tissue regeneration by transplantation of osteoprotegerin-engineered periodontal ligament stem cells." in: Stem cell research & therapy, Vol. 6, pp. 22, (2015) (PubMed).

Details zu Vinculin

Target: Vinculin (VCL)

Andere Bezeichnung: VCL

Hintergrund:

Synonyms: Vinculin,Metavinculin,MV,VCL,

Tissue Specificity: Metavinculin is muscle-specific.

Background: Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined.

Molekulargewicht: 124 kDa

UniProt: P18206

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2