Choline Acetyltransferase Antikörper (AA 446-652)

Produktnummer ABIN5518818

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Choline Acetyltransferase in WB und IHC. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte und wurde in 7+ Publikationen erwähnt.

Kurzübersicht für Choline Acetyltransferase Antikörper (AA 446-652) (ABIN5518818)

Target: Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Choline Acetyltransferase Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-Choline Acetyltransferase Antikörper

Bindungsspezifität: AA 446-652

Verwendungszweck: Anti-Choline Acetyltransferase/CHAT Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-Choline Acetyltransferase/CHAT Antibody Picoband® (ABIN5518818). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human CHAT recombinant protein (Position: D446-R652). Human CHAT shares 88.4% and 87% amino acid (aa) sequence identity with mouse and rat CHAT, respectively.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Mouse, Rat, Human
Western blot, 0.1-0.5 μg/mL, Mouse, Rat, Human
1. Berman R, Wilson IB, Nachmansohn D (September-October 1953). "Choline acetylase specificity in relation to biological function.". Biochimica et Biophysica Acta. 12 (1-2): 315-24. 2. Strauss WL, Kemper RR, Jayakar P, Kong CF, Hersh LB, Hilt DC, Rabin M (February 1991). "Human choline acetyltransferase gene maps to region 10q11-q22.2 by in situ hybridization". Genomics. 9 (2): 396-8.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Referenzen für anti-Choline Acetyltransferase Antikörper (ABIN5518818)

Yu, Xia, Tang, Xu, Wei, Chen, Dai, Gong, Bi: "Acetylcholine acts through M3 muscarinic receptor to activate the EGFR signaling and promotes gastric cancer cell proliferation." in: Scientific reports, Vol. 7, pp. 40802, (2018) (PubMed).

Zhao, Xu, Pan, Zhu, Fu, Guo, Lu, Wang: "Pharmacologic activation of cholinergic alpha7 nicotinic receptors mitigates depressive-like behavior in a mouse model of chronic stress." in: Journal of neuroinflammation, Vol. 14, Issue 1, pp. 234, (2018) (PubMed).

Hu, Wang, Ren, Liu: "Autonomic remodeling may be responsible for decreased incidence of aortic dissection in STZ-induced diabetic rats via down-regulation of matrix metalloprotease 2." in: BMC cardiovascular disorders, Vol. 16, Issue 1, pp. 200, (2017) (PubMed).

Wang, He, Zhong, Lv, Xiao, Liu: "Overexpression of NTRK1 Promotes Differentiation of Neural Stem Cells into Cholinergic Neurons." in: BioMed research international, Vol. 2015, pp. 857202, (2016) (PubMed).

Liu, Deng, Zhang, Zhang: "Wnt3a expression during the differentiation of adipose-derived stem cells into cholinergic neurons." in: Neural regeneration research, Vol. 7, Issue 19, pp. 1463-8, (2015) (PubMed).

Zhang, Li, Gu, Liu, Xu, Cui, Sun: "Thalidomide influences growth and vasculogenic mimicry channel formation in melanoma." in: Journal of experimental & clinical cancer research : CR, Vol. 27, pp. 60, (2009) (PubMed).

Zhang, Qi, Li, Zhang, Xu, Wang, Sun: "Chemokine CXCL12 and its receptor CXCR4 expression are associated with perineural invasion of prostate cancer." in: Journal of experimental & clinical cancer research : CR, Vol. 27, pp. 62, (2009) (PubMed).

Details zu Choline Acetyltransferase

Target: Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

Andere Bezeichnung: CHAT

Hintergrund:

Synonyms: Choline O-acetyltransferase,CHOACTase,ChAT,Choline acetylase,2.3.1.6,CHAT,

Background: Choline acetyltransferase (commonly abbreviated as ChAT, but sometimes CAT) is a transferase enzyme responsible for the synthesis of the neurotransmitter acetylcholine. In humans, the choline acetyltransferase enzyme is encoded by the CHAT gene. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform.

Molekulargewicht: 83 kDa

Gen-ID: 1103

UniProt: P28329

Pathways: Skeletal Muscle Fiber Development