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PDE5A Antikörper (N-Term)

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch PDE5A in WB und IHC. Er zeigt eine Reaktivität gegenüber Human und Ratte.
Produktnummer ABIN5518785

Kurzübersicht für PDE5A Antikörper (N-Term) (ABIN5518785)

Target

Alle PDE5A Antikörper anzeigen
PDE5A (phosphodiesterase 5A, cGMP-Specific (PDE5A))

Reaktivität

  • 28
  • 8
  • 7
  • 4
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Human, Ratte

Wirt

  • 23
  • 4
  • 1
Kaninchen

Klonalität

  • 25
  • 3
Polyklonal

Konjugat

  • 18
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser PDE5A Antikörper ist unkonjugiert

Applikation

  • 21
  • 13
  • 12
  • 11
  • 9
  • 7
  • 4
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (IHC)
  • Bindungsspezifität

    • 6
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    AA 20-63, N-Term

    Verwendungszweck

    Anti-PDE5A Antibody Picoband®

    Sequenz

    QKQQQRDQDS VEAWLDDHWD FTFSYFVRKA TREMVNAWFA ERVH

    Kreuzreaktivität (Details)

    No cross-reactivity with other proteins

    Produktmerkmale

    Anti-PDE5A Antibody Picoband® (ABIN5518785). Tested in IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    Aufreinigung

    Immunogen affinity purified.

    Immunogen

    A synthetic peptide corresponding to a sequence at the N-terminus of human PDE5A, different from the related mouse sequence by eight amino acids.

    Isotyp

    IgG
  • Applikationshinweise

    Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
    Western blot, 0.1-0.5 μg/mL, Human, Rat
    1. Gebska, M. A., Stevenson, B. K., Hemnes, A. R., Bivalacqua, T. J., Haile, A., Hesketh, G. G., Murray, C. I., Zaiman, A. L., Halushka, M. K., Krongkaew, N., Strong, T. D., Cooke, C. A., El-Haddad, H., Tuder, R. M., Berkowitz, D. E., Champion, H. C.Phosphodiesterase-5A (PDE5A) is localized to the endothelial caveolae and modulates NOS3 activity. Cardiovasc. Res. 90: 353-363, 2011. 2. Lin, C.-S., Chow, S., Lau, A., Tu, R., Lue, T. F. Human PDE5A gene encodes three PDE5 isoforms from two alternate promoters.Int. J. Impotence Res. 14: 15-24, 2002. 3. Loughney, K., Hill, T. R., Florio, V. A., Uher, L., Rosman, G. J., Wolda, S. L., Jones, B. A., Howard, M. L., McAllister-Lucas, L. M., Sonnenburg, W. K., Francis, S. H., Corbin, J. D., Beavo, J. A., Ferguson, K. Isolation and characterization of cDNAs encodingPDE5A, a human cGMP-binding, cGMP-specific 3-prime,5-prime-cyclic nucleotide phosphodiesterase. Gene 216: 139-147, 1998.

    Kommentare

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    Konzentration

    500 μg/mL

    Buffer

    Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • Target

    PDE5A (phosphodiesterase 5A, cGMP-Specific (PDE5A))

    Andere Bezeichnung

    PDE5A

    Hintergrund

    Synonyms: cGMP-specific 3',5'-cyclic phosphodiesterase,3.1.4.35 ,cGMP-binding cGMP-specific phosphodiesterase,CGB-PDE,PDE5A,PDE5,

    Tissue Specificity: Expressed in aortic smooth muscle cells, heart, placenta, skeletal muscle and pancreas and, to a much lesser extent, in brain, liver and lung.

    Background: cGMP-specific phosphodiesterase type 5 is an enzyme from the phosphodiesterase class. It is found in various tissues, most prominently the corpus cavernosum and the retina. It has also been recently discovered to play a vital role in the cardiovascular system. Furthermore, PDE5A also plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This PDE5A gene is mapped to 4q26.

    Molekulargewicht

    100 kDa

    Gen-ID

    8654

    UniProt

    O76074

    Pathways

    Regulation of G-Protein Coupled Receptor Protein Signaling
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