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HSD17B1 Antikörper (N-Term)

Dieses Anti-HSD17B1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von HSD17B1 in WB, IHC und ICC. Geeignet für Human und Ratte. Dieses Primary Antibody wurde in 1 Publikation zitiert.
Produktnummer ABIN5518679

Kurzübersicht für HSD17B1 Antikörper (N-Term) (ABIN5518679)

Target

Alle HSD17B1 Antikörper anzeigen
HSD17B1 (Hydroxysteroid (17-Beta) Dehydrogenase 1 (HSD17B1))

Reaktivität

  • 60
  • 20
  • 19
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Human, Ratte

Wirt

  • 59
  • 3
  • 1
Kaninchen

Klonalität

  • 60
  • 3
Polyklonal

Konjugat

  • 25
  • 7
  • 4
  • 4
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser HSD17B1 Antikörper ist unkonjugiert

Applikation

  • 43
  • 20
  • 16
  • 9
  • 8
  • 5
  • 4
  • 4
  • 2
  • 1
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
  • Bindungsspezifität

    • 9
    • 6
    • 5
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    AA 29-43, N-Term

    Verwendungszweck

    Anti-HSD17B1 Antibody Picoband®

    Sequenz

    QSFKVYATLR DLKTQ

    Kreuzreaktivität (Details)

    No cross-reactivity with other proteins

    Produktmerkmale

    Anti-HSD17B1 Antibody (ABIN5518679). Tested in IHC, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    Aufreinigung

    Immunogen affinity purified.

    Immunogen

    A synthetic peptide corresponding to a sequence at the N-terminus of human HSD17B1, different from the related rat and mouse sequences by one amino acid.

    Isotyp

    IgG
  • Applikationshinweise

    Western blot, 0.1-0.5 μg/mL, Human, Rat
    Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat
    Immunocytochemistry , 0.5-1 μg/mL, Human
    1. "Entrez Gene: HSD17B1 Hydroxysteroid (17-beta) dehydrogenase 1". 2. Aka JA, Mazumdar M, Chen CQ, Poirier D, Lin SX (Apr 2010). "17beta-hydroxysteroid dehydrogenase type 1 stimulates breast cancer by dihydrotestosterone inactivation in addition to estradiol production".Molecular Endocrinology. 24 (4): 832-45.

    Kommentare

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    Konzentration

    500 μg/mL

    Buffer

    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

    Konservierungsmittel

    Sodium azide, Thimerosal (Merthiolate)

    Vorsichtsmaßnahmen

    This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • Li, Peng, Zeng, Liu, Hao, Yu, Zhu, Hu: "Estrogen Secreted by Mesenchymal Stem Cells Necessarily Determines Their Feasibility of Therapeutical Application." in: Scientific reports, Vol. 5, pp. 15286, (2016) (PubMed).

  • Target

    HSD17B1 (Hydroxysteroid (17-Beta) Dehydrogenase 1 (HSD17B1))

    Andere Bezeichnung

    HSD17B1

    Hintergrund

    Synonyms: Estradiol 17-beta-dehydrogenase 1,1.1.1.62,17-beta-hydroxysteroid dehydrogenase type 1,17-beta-HSD 1,20 alpha-hydroxysteroid dehydrogenase,20-alpha-HSD,E2DH,Placental 17-beta-hydroxysteroid dehydrogenase,Short chain dehydrogenase/reductase family 28C member 1,HSD17B1,E17KSR, EDH17B1, EDH17B2, EDHB17, SDR28C1,

    Tissue Specificity: Detected in liver, small intestine, spleen, placenta, thymus, lung, brain, kidney, and colon. .

    Background: Estradiol 17-beta-dehydrogenase 1 is an enzyme that in humans is encoded by the HSD17B1 gene. This gene encodes a member of the 17beta-hydroxysteroid dehydrogenase family of short-chain dehydrogenases/reductases. It has a dual function in estrogen activation and androgen inactivation and plays a major role in establishing the estrogen E2 concentration gradient between serum and peripheral tissues. The encoded protein catalyzes the last step in estrogen activation, using NADPH to convert estrogens E1 and E2 and androgens like 4-androstenedione, to testosterone. It has an N-terminal short-chain dehydrogenase domain with a cofactor binding site, and a narrow, hydrophobic C-terminal domain with a steroid substrate binding site. This gene is expressed primarily in the placenta and ovarian granulosa cells, and to a lesser extent, in the endometrium, adipose tissue, and prostate. Polymorphisms in this gene have been linked to breast and prostate cancer. A pseudogene of this gene has been identified. Alternative splicing results in multiple transcript variants.

    Sequence Similarities: Belongs to the short-chain dehydrogenases/reductases (SDR) family.

    Molekulargewicht

    37-38 kDa

    UniProt

    P14061

    Pathways

    Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis
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