SUPT5H Antikörper (N-Term)

Produktnummer ABIN5514714

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch SUPT5H in WB. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für SUPT5H Antikörper (N-Term) (ABIN5514714)

Target: SUPT5H (Transcription Elongation Factor SPT5 (SUPT5H))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SUPT5H Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-SUPT5H Antikörper

Bindungsspezifität: N-Term

Sequenz: SAAGSEKEEE PEDEEEEEEE EEYDEEEEEE DDDRPPKKPR HGGFILDEAD

Aufreinigung: Affinity purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human SUPT5H

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Details zu SUPT5H

Target: SUPT5H (Transcription Elongation Factor SPT5 (SUPT5H))

Andere Bezeichnung: SUPT5H

Hintergrund: Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences.

Alias Symbols: SPT5, SPT5H, Tat-CT1

Protein Size: 1087

Gen-ID: 6829

NCBI Accession: NM_001111020, NP_001104490

UniProt: O00267