SCNN1A Antikörper (Sodium Channel, Nonvoltage-Gated 1 alpha) (AA 46-68) (APC)

Details for Product anti-SCNN1A Antibody No. ABIN5066867
Antigen
Epitop
AA 46-68
127
40
36
25
25
10
8
8
6
3
2
2
1
1
1
1
1
1
1
Reaktivität
Maus, Ratte (Rattus)
191
188
130
54
50
38
Wirt
Maus
155
85
1
Klonalität (Klon)
Monoklonal ()
Konjugat
Dieser SCNN1A Antikörper ist konjugiert mit APC
11
10
10
10
10
9
8
8
8
8
8
8
8
8
8
8
8
5
5
5
5
5
5
1
1
1
1
1
1
Applikation
Immunohistochemistry (IHC), Western Blotting (WB)
223
197
55
49
43
41
28
9
5
3
2
1
Optionen
Immunogen Synthetic peptide from the N-terminal of Rat ENaC alpha (aa. 46-68)
Klon 2G4
Isotyp IgG2b
Spezifität Detects ~85 kDa.
Reinigung Protein G Purified
Antigen
Andere Bezeichnung ENaC alpha (SCNN1A Antibody Abstract)
Hintergrund The Epithelial Sodium Channel (ENaC) is a membrane ion channel permeable to Na+ ions. It is located in the apical plasma membrane of epithelia in the kidneys, lung, colon, and other tissues where it plays a role in trans epithelial Na+-ion transport (1). Specifically Na+ transport via ENaC occurs across many epithelial surfaces, and plays a key role in regulating salt and water absorption (2). ENaCs are composed of three structurally related subunits that form a tetrameric channel, alpha, beta, and gamma. The expression of its alpha and beta subunits is enhanced as keratinocytes differentiate (3, 4). The beta and gamma-ENaC subunits are essential for edema fluid to exert its maximal effect on net fluid absorption by distal lung epithelia(5). And it has been concluded that the subunits are differentially expressed in the retina of mice with ocular hypertension, therefore the up-regulation of alpha-ENaC proteins could serve as a protection mechanism against elevated intraocular pressure (6).
Gen-ID 25122
NCBI Accession NP_113736
UniProt Q6IRJ1
Applikations-hinweise
  • WB (1:1000)
  • IHC (1:150)
  • optimal dilutions for assays should be determined by the user.
Kommentare

A 1:1000 dilution of ABIN5066867 was sufficient for detection of ENaC alpha in 15 μg of Mouse whole kidney lysate by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Bilder des Herstellers
Image no. 1 for anti-Sodium Channel, Nonvoltage-Gated 1 alpha (SCNN1A) (AA 46-68) antibody (APC) (ABIN5066867) Immunohistochemistry analysis using Mouse Anti-ENaC alpha Monoclonal Antibody, Clone ...
Image no. 2 for anti-Sodium Channel, Nonvoltage-Gated 1 alpha (SCNN1A) (AA 46-68) antibody (APC) (ABIN5066867) Immunohistochemistry analysis using Mouse Anti-ENaC alpha Monoclonal Antibody, Clone ...
Image no. 3 for anti-Sodium Channel, Nonvoltage-Gated 1 alpha (SCNN1A) (AA 46-68) antibody (APC) (ABIN5066867) Western Blot analysis of Mouse Whole kidney homogenates showing detection of ~85kDa E...