Ubiquitin Antikörper (Biotin)

Produktnummer ABIN5024521

Dieses Anti-Ubiquitin-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Ubiquitin in WB, IHC, ELISA, ICC, IF und IP. Geeignet für Human.

Kurzübersicht für Ubiquitin Antikörper (Biotin) (ABIN5024521)

Target: Ubiquitin (Ub)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Ubiquitin Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)

Klon: FK2

Produktdetails anti-Ubiquitin Antikörper (Biotin)

Spezifität: Detects ubiquitinated proteins and ubiquitin chains. Does not detect free ubiquitin.

Aufreinigung: Protein A Purified

Immunogen: Ubiquitin conjugated lysozyme

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

• WB (1:10000)
• optimal dilutions for assays should be determined by the user.

Kommentare:

A 1:5000 dilution of ABIN5024521 was sufficient for detection of ubiquitin conjugates in 2.5 μg of HeLa cell lysate by electrochemiluminescence analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 10 mg/mL

Buffer: 10 mM phosphate buffer, 0.15M NaCl pH 7.4, 0.1 % sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Conjugated antibodies should be stored at 4°C

Details zu Ubiquitin

Target: Ubiquitin (Ub)

Andere Bezeichnung: Ubiquitin

Hintergrund: Ubiquitin is a small protein found ubiquitously in all tissue types and acts as a post translational modification. It can bind to its substrate either as a single ubiquitin molecule or in a chain. It is involved in many regulatory processes, which include proteasomal degradation, signal transduction, DNA repair, endocytosis and autophagy.

Gen-ID: 7314

UniProt: P0CG47

Pathways: Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway