TSC1 Antikörper (both Isoforms, Middle Region)

Produktnummer ABIN501071

Der Kaninchen Polyklonal anti-TSC1 Antikörper wird verwendet zum Nachweis von TSC1 in Proben von Human, Maus und Ratte. Er wurde validiert für WB, IF und EIA.

Kurzübersicht für TSC1 Antikörper (both Isoforms, Middle Region) (ABIN501071)

Target: TSC1 (Tuberous Sclerosis 1 (TSC1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TSC1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Produktdetails anti-TSC1 Antikörper

Bindungsspezifität: both Isoforms, Middle Region

Spezifität: This antibody detects TSC1 / Hamartin at Center.

Kreuzreaktivität (Details): Species reactivity (tested):Human, mouse, rat

Aufreinigung: Peptide affinity chromatography

Immunogen: TSC1 antibody was raised against a 15 amino acid peptide from the middle region of human TSC1. TSC1 antibody will recognize both isoforms of TSC1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA. Western blot: 1 μg/mL. Immunoflourescence.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS containing 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Details zu TSC1

Target: TSC1 (Tuberous Sclerosis 1 (TSC1))

Andere Bezeichnung: TSC1 / Hamartin

Hintergrund: Tuberous sclerosis complex (TSC) is an autosomal dominant tumor syndrome caused by mutations in either of the TSC1 or TSC2 tumor suppressor genes. The products of these genes form a protein complex that indirectly decreases the signaling of the mammalian Target of Rapamycin (TOR), an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors (reviewed in 1,2). TOR activity is stimulated by Rheb, a member of the Ras superfamily of G-proteins, when the GTP/GDP ratio bound to Rheb is high (3). Immunoprecipitated TSC1/TSC2 has been shown to stimulate Rheb GTPase activity in vitro (4), suggesting that the TSC1/TSC2 decreases the ability of Rheb to stimulate TOR activity. This is supported by experiments showing overexpression of TSC1 and TSC2 results in a significant decrease in the GTP/GDP ratio bound to Rheb and the inhibition of cell growth (4,5). A shorter 40 kDa isoform of TSC1 has been shown to exist but its function is unknown.Synonyms: KIAA0243, TSC, Tuberous sclerosis 1 protein

Gen-ID: 7248

Pathways: RTK Signalweg, AMPK Signaling, Regulation of Cell Size, Tube Formation