FAM33A Antikörper (N-Term)

Produktnummer ABIN500730

Produktdetails anti-FAM33A Antikörper

Target: FAM33A (Spindle and Kinetochore-Associated Protein 2 (FAM33A))

Bindungsspezifität: N-Term

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser FAM33A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Spezifität: This antibody detects SKA2.

Kreuzreaktivität (Details): Species reactivity (tested):Human, mouse

Aufreinigung: Peptide affinity chromatography

Immunogen: SKA2 antibody was raised against a 15 amino acid peptide from near the amino terminus of human SKA2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA. Western blot: 0.5 - 1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS containing 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Details zu FAM33A

Target: FAM33A (Spindle and Kinetochore-Associated Protein 2 (FAM33A))

Andere Bezeichnung: SKA2 (FAM33A Produkte)

Synonyme: FAM33A antikoerper, 1110001A07Rik antikoerper, C78640 antikoerper, Fam33a antikoerper, RGD1307084 antikoerper, fam33a antikoerper, spindle and kinetochore associated complex subunit 2 antikoerper, Spindle and kinetochore-associated protein 2 antikoerper, SKA2 antikoerper, Ska2 antikoerper, ska2 antikoerper

Hintergrund: Upon entry into mitosis, the cell's microtubule (MT) network forms the mitotic spindle, allowing the segregation of paired chromosomes. Proteinaceous structures on centromeric chromatin termed kinetochores (KT) are essential for the proper attachment of the chromosomes to the spindle MTs. A recently discovered spindle and kinetochore complex, comprised of proteins SKA1, SKA2, and SKA3, has been found to be required for stable KT-MT interactions and timely anaphase onset. Depletion of either SKA1 or SKA2 by siRNA results in the loss of both proteins from the KT, but does not impact overall KT structure. Cells depleted of the SKA complex undergo a prolonged checkpoint-dependent delay in a metaphase-like state, indicating the importance of the SKA complex in the maintenance of the metaphase plate and spindle checkpoint silencing. SKA2 has also been shown to interact with glucocorticoid receptors and to be involved in glucocorticoid signaling and cell proliferation.Synonyms: FAM33A, Spindle and kinetochore-associated protein 2

Gen-ID: 348235

NCBI Accession: NP_872426

UniProt: Q8WVK7

Pathways: M Phase