RNF168 Antikörper (C-Term)

Produktnummer ABIN500624

Der Kaninchen Polyklonal Anti-RNF168-Antikörper wurde für WB und EIA validiert. Er ist geeignet, RNF168 in Proben von Human und Maus zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für RNF168 Antikörper (C-Term) (ABIN500624)

Target: RNF168 (Ring Finger Protein 168 (RNF168))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RNF168 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-RNF168 Antikörper

Bindungsspezifität: C-Term

Spezifität: This antibody detects RNF168.

Kreuzreaktivität (Details): Species reactivity (tested):Human, mouse

Aufreinigung: Peptide affinity chromatography

Immunogen: RNF168 antibody was raised against an 18 amino acid peptide from near the carboxy terminus of human RNF168.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA. Western blot: 1 - 2 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS containing 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Referenzen für anti-RNF168 Antikörper (ABIN500624)

Li, Guan, Huang, Hu, Zheng: "RNF168-mediated H2A neddylation antagonizes ubiquitylation of H2A and regulates DNA damage repair." in: Journal of cell science, Vol. 127, Issue Pt 10, pp. 2238-48, (2014) (PubMed).

Details zu RNF168

Target: RNF168 (Ring Finger Protein 168 (RNF168))

Andere Bezeichnung: RNF168

Hintergrund: RNF168 was identified as a chromatin-associated RING finger protein that acts as a ubiquitin ligase both in vitro and in vivo. RNF168 targets histones H2A and H2AX, but not H2B, forming K63 polyubiquitin chains. Upon formation of DNA double strand breaks, RNF168 is recruited to the site of DNA damage where it co-localizes with gammaH2AX and 53BP1 in an RNF8-dependent manner. This localization of RNF168 increases the local concentration of ubiquinated proteins to the threshold required for retention of the proteins 53BP1 and BRCA1, facilitating the downstream signaling cascade. Thus, RNF168 defines a new pathway demonstrating a functional cooperation between E3 ligases in genome maintenance. At least three isoforms of RNF168 are known to exist.Synonyms: E3 ubiquitin-protein ligase RNF168, RING finger protein 168

Gen-ID: 165918

NCBI Accession: NP_689830

UniProt: Q8IYW5

Pathways: Production of Molecular Mediator of Immune Response