POFUT1 Antikörper (N-Term)

Produktnummer ABIN500521

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch POFUT1 in WB, IHC (p) und EIA. Er zeigt eine Reaktivität gegenüber Human und Maus und wurde in 1 Publikation erwähnt.

Kurzübersicht für POFUT1 Antikörper (N-Term) (ABIN500521)

Target: POFUT1 (Protein O-Fucosyltransferase 1 (POFUT1))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser POFUT1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-POFUT1 Antikörper

Bindungsspezifität: N-Term

Spezifität: This antibody detects POFUT1. It is predicted to not cross-react with POFUT2.

Kreuzreaktivität (Details): Species reactivity (tested):Human, mouse

Aufreinigung: Peptide affinity chromatography

Immunogen: POFUT1 antibody was raised against a 16 amino acid peptide from near the amino terminus of human POFUT1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA. Western blot: .5 - 1 μg/mL. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS containing 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Referenzen für anti-POFUT1 Antikörper (ABIN500521)

Takeuchi, Wong, Schneider, Freeze, Takeuchi, Berardinelli, Ito, Lee, Nelson, Haltiwanger: "Variant in human POFUT1 reduces enzymatic activity and likely causes a recessive microcephaly, global developmental delay with cardiac and vascular features." in: Glycobiology, Vol. 28, Issue 5, pp. 276-283, (2018) (PubMed).

Details zu POFUT1

Target: POFUT1 (Protein O-Fucosyltransferase 1 (POFUT1))

Andere Bezeichnung: POFUT1

Hintergrund: POFUT1, an endoplasmic reticulum-residing member of the glycosyltransferase O-Fuc family, adds O-fucose through an O-glycosidic linkage to conserved serine or threonines in epidermal growth factor-like repeats of several cell surface and secreted proteins. Unlike its homolog POFUT2, POFUT1 can also catalyze the transfer of fucose to thrombospondin type 1 repeats. Many of the substrates of POFUT1 are involved in ligand-induced receptor signaling. One such protein is Notch, mouse ES cells lacking POFUT have normal levels of Notch receptors at the cell surface, but these receptors do not bind Notch ligands or exhibit Notch signaling. At least two isoforms of POFUT1 are known to exist.Synonyms: FUT12, GDP-fucose protein O-fucosyltransferase 1, KIAA0180, O-FucT-1, Peptide-O-fucosyltransferase 1

Gen-ID: 23509

NCBI Accession: NP_056167

UniProt: Q9H488

Pathways: Notch Signalweg, SARS-CoV-2 Protein Interaktom