PIAS1 Antikörper (C-Term)

Produktnummer ABIN500489

Der Kaninchen Polyklonal Anti-PIAS1-Antikörper wurde für WB, IHC (p), IF und EIA validiert. Er ist geeignet, PIAS1 in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für PIAS1 Antikörper (C-Term) (ABIN500489)

Target: PIAS1 (Protein Inhibitor of Activated STAT, 1 (PIAS1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PIAS1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Produktdetails anti-PIAS1 Antikörper

Bindungsspezifität: C-Term

Spezifität: This antibody is predicted to not cross-react with other members of the PIAS family.

Kreuzreaktivität (Details): Species reactivity (tested):Human, Mouse, Rat.

Aufreinigung: Affinity chromatography purified via peptide column

Immunogen: 15 amino acid peptide near the carboxy terminus of Human PIAS1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA. Western blot: 1 - 2 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS containing 0.02 % Sodium Azide as preservative

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu PIAS1

Target: PIAS1 (Protein Inhibitor of Activated STAT, 1 (PIAS1))

Andere Bezeichnung: PIAS1

Hintergrund: The PIAS proteins (protein inhibitor of activated STAT) play a crucial role as transcriptional coregulators in various cellular pathways, including the STAT, p53 and the steroid hormone signaling pathway. The PIAS protein family includes at least five evolutionarily conserved genes, including PIAS1. The major function of the PIAS proteins is the control of gene transcription and can also act as small ubiquitin-like-modifier (SUMO) E3 ligases. PIAS1 binds specifically to STAT1, inhibiting STAT1-mediated gene activation and also binds to the Gu/RNA helicase II enzyme, leading to the proteolytic cleavage of Gu/RH-II. PIAS1 is a potent co-activator for CP2c-mediated ?-globin expression in erythroid cells.Synonyms: DDXBP1, DEAD/H box-binding protein 1, E3 SUMO-protein ligase PIAS1, Gu-binding protein, PIAS-1, Protein inhibitor of activated STAT protein 1, RNA helicase II-binding protein

Gen-ID: 8554

NCBI Accession: NP_057250

UniProt: O75925

Pathways: JAK-STAT Signalweg, Interferon-gamma Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Muscle Cell Differentiation, Hepatitis C