Der Maus Monoklonal anti-HA-Tag Antikörper (Klon 7H5) (ABIN5004206) detektiert spezifisch HA-Tag in WB, IP und IF (cc).
Dieser Antikörper reagiert spezifisch mit Proben aus Human und Diverse Spezies.
Animal-Free
Reaktivität: Tag
WB, ELISA, IF
Wirt: Maus
Monoclonal
AB158-1-D11
unconjugated
Recombinant Antibody
Applikationshinweise
WB 1:300-5000 IF(ICC) 1:50-200 IP(1-2 μg)
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Buffer
0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
Konservierungsmittel
ProClin
Vorsichtsmaßnahmen
This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Lagerung
4 °C,-20 °C
Informationen zur Lagerung
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Haltbarkeit
12 months
Target
HA-Tag
Andere Bezeichnung
HA tag
Substanzklasse
Tag
Hintergrund
Optional[synonyms]: Plasmid vectors for the expression of coding regions of eukaryotic genes in bacterial, insect and mammalian hosts are in common usage. The expression vectors are used to encode hybrid fusion proteins consisting of a eukaryotic target protein and a specialized region designed to aid in the purification and visualization of the target protein. Short pieces of welldefined peptides(Poly-His, Flag-epitope or cmyc epitope or HA-tag) or small proteins (bacterial GST, MBP) is often cloned along with the target gene. The HA tag is derived from an epitope of the influenza hemagglutinin protein, which has been extensively used as a general epitope tag in expression vectors. It has been successfully used in a variety of applications, including Western blotting, Immunoprecipitation, and immunofluorescence studies.