ERN1 Antikörper (C-Term)

Produktnummer ABIN500048

Der Kaninchen Polyklonal Anti-ERN1-Antikörper wurde für WB, IF und EIA validiert. Er ist geeignet, ERN1 in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für ERN1 Antikörper (C-Term) (ABIN500048)

Target: ERN1 (Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ERN1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Produktdetails anti-ERN1 Antikörper

Bindungsspezifität: C-Term

Spezifität: IRE1p antibody was raised against a 16 amino acid peptide from near the carboxy terminus of human IRE1p.

Aufreinigung: Affinity Chromatography purified via peptide column

Immunogen: 16 amino acid synthetic peptide from near the carboxy terminus of Human IRE1p

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA. Western Blot: 1 - 2 μg/mL. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS containing 0.02 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu ERN1

Target: ERN1 (Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1))

Andere Bezeichnung: ERN1 / IRE1

Hintergrund: Accumulation of malfolded proteins in the endoplasmic reticulum (ER) activates the unfolded protein response (UPR) and the upregulation of the ER molecular chaperones GRP78 and GRP 94 (1,2). These proteins are normally bound to ER transmembrane proteins such as IRE1p and ATF6 (3,4) but ER stress causes their dissociation. This allows IRE1p, a serine-threonine protein kinase to transduce the unfolded protein signal from the ER to the nucleus. IRE1p also has an endoribonuclease activity that is required to splice X-box binding protein (XBP1) mRNA converting it to a potent UPR transcriptional activation (5). Depletion of IRE1p through the expression of a dominant negative form of IRE1p has no effect on transfected cells, but cell death via apoptosis occurs under stress conditions that cause unfolded proteins to accumulate in the ER (6). Two alternatively spliced transcript variants encoding different isoforms have been found for this gene.Synonyms: Endoplasmic reticulum-to-nucleus signaling 1, IRE1a, IRE1p, Inositol-requiring protein 1, Ire1-alpha, Serine/threonine-protein kinase/endoribonuclease IRE1

Gen-ID: 2081

UniProt: O75460

Pathways: ER-Nucleus Signaling, Unfolded Protein Response