BrdU Antikörper (AbBy Fluor® 750)

Produktnummer ABIN4997617

Der Kaninchen Polyklonal Anti-BrdU-Antikörper wurde für IF (cc) und IF (p) validiert. Er ist geeignet, BrdU in Proben von zu detektieren.

Kurzübersicht für BrdU Antikörper (AbBy Fluor® 750) (ABIN4997617)

Target: BrdU (Bromodeoxyuridine (BrdU))

Reaktivität: Bitte anfragen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser BrdU Antikörper ist konjugiert mit AbBy Fluor® 750

Applikation: Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Produktdetails anti-BrdU Antikörper (AbBy Fluor® 750)

Kreuzreaktivität: Human, Ratte

Kreuzreaktivität (Details): BrdU

Aufreinigung: Purified by Protein A.

Immunogen: BrdU conjugated to KLH

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IF(IHC-P) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu BrdU

Target: BrdU (Bromodeoxyuridine (BrdU))

Andere Bezeichnung: BrdU

Substanzklasse: Chemical

Hintergrund:

Synonyms: Bromodeoxyuridine, Bromodeoxyuridine, 5-Bromo-2-deoxyuridine, 5-BrdU, proliferation Marker.

Background: The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal is an accurate and comprehensive method to quantitate the degree of DNA-synthesis.BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis