DFFA Antikörper (N-Term)

Produktnummer ABIN499744

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch DFFA in WB und EIA. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für DFFA Antikörper (N-Term) (ABIN499744)

Target: DFFA (DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DFFA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-DFFA Antikörper

Bindungsspezifität: N-Term

Spezifität: DFF45 antibody was raised against a peptide corresponding to amino acids 2 to 21 of human DFF45.

Aufreinigung: DEAE

Immunogen: Human DFF45 (N-Terminus) Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA. Western Blot: DFF45 antibody can be used for detection of DFF45, DFF35, and one thecleaved fragment at 1/1000 to 1/2000 dilution.45 and 35 kDa bands can be detected innon-apoptotic cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C.

Details zu DFFA

Target: DFFA (DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA))

Andere Bezeichnung: DFFA / ICAD

Hintergrund: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A human 45 kDa DNA fragmentation factor (DFF45) was identified recently that was cleaved by caspase-3 during apoptosis. Mouse homologue of human DFF45 was identified as a DNase inhibitor designated ICAD. DFF45/ICAD have short forms that were termed DFF35 and ICADs, respectively. Upon cleavage of DFF45/ICAD, the caspase activated deoxyribonuclease (DFF40/CAD) is released and activated and eventually causes the degradation of DNA in the nuclei. Therefore, the cleavage of DFF45/ICAD, which causes DFF40/CAD activation and DNA degradation, is the hallmark of apoptotic cell death.Synonyms: DFF-45, DFF1, DFF45, DNA fragmentation factor 45 kDa subunit, DNA fragmentation factor subunit alpha, Inhibitor of CAD

Gen-ID: 1676

NCBI Accession: NP_004392

UniProt: O00273

Pathways: Apoptose, Caspase Kaskade in der Apoptose