APAF1 Antikörper (N-Term)

Produktnummer ABIN499287

Dieses Anti-APAF1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von APAF1 in WB, IHC (p) und EIA. Geeignet für Human, Maus und Ratte.

Kurzübersicht für APAF1 Antikörper (N-Term) (ABIN499287)

Target: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser APAF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-APAF1 Antikörper

Bindungsspezifität: N-Term

Spezifität: Apaf1 antibody was raised against a peptide corresponding to amino acids near the amino terminus of human Apaf1. The sequences of the immunogenic peptide are identical between human and mouse.

Aufreinigung: Affinity chromatography purified via peptide column

Immunogen: Human Apaf1 (N-Terminus) Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA. Western Blot: Apaf1 antibody can be used for detection of Apaf1 at 1 - 2 μg/mL. Whole celllysate from HeLa cells can be used as positive control and a 115 - 130 kDa band should bedetected. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C.

Details zu APAF1

Target: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Andere Bezeichnung: APAF1

Hintergrund: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. The mammalian homologues of the key cell death gene CED-4 in C. elegans has been identified recently from human and mouse and designated Apaf1 (for apoptosis protease-activating factor 1) (1,2). Apaf1 binds to cytochrome c (Apaf-2) and caspase-9 (Apaf-3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis (3). Apaf1 can also associate with caspase-4 and caspase-8 (4). Apaf1 is ubiquitously expressed in human tissues .Synonyms: Apaf-1, Apoptotic protease-activating factor 1, KIAA0413

Gen-ID: 317

UniProt: O14727

Pathways: p53 Signalweg, Apoptose, Caspase Kaskade in der Apoptose, Tube Formation, Positive Regulation of Endopeptidase Activity