AES Antikörper (C-Term, N-Term)

Produktnummer ABIN499225

Produktdetails anti-AES Antikörper

Target: AES (Amino-terminal Enhancer of Split (AES))

Bindungsspezifität: C-Term, N-Term

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser AES Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Spezifität: AES antibody was raised against a 16 amino acid peptide from near the carboxy terminus of human AES.

Aufreinigung: Affinity chromatography purified via peptide column

Immunogen: Human AES / Amino-terminal enhancer of split / GRG / ESP1 / TLE5 (C-Terminus) Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA. Western Blot: AES antibody can be used for the detection of AES at 1 - 2 μg/mL. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS containing 0.02 % Sodium Azide as preservative

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu AES

Target: AES (Amino-terminal Enhancer of Split (AES))

Andere Bezeichnung: AES / ESP1 (AES Produkte)

Hintergrund: Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apop-tosis induced by integrin detachment (1). Amino-terminal enhancer of split (AES) is a member of the Groucho/ transducin-like enhancer of split (TLE) family of transcriptional regulators, a group of transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination (2,3). AES forms a complex with Bit1 (Bcl-2 inhibitor of transcription 1), a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis (4). It has been suggested that this complex turns off a survival-promoting gene transcription program controlled by the TLE protein family. (4). Interestingly, apoptosis of cells transfected with AES and Bit1 could be inhibited if the cells were allowed to attach to fibronectin through the alpha5beta1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the alpha5beta1 integrin (4).Synonyms: AES1, Amino-terminal enhancer of split, GRG protein, Gp130-associated protein GAM

Gen-ID: 166

NCBI Accession: NP_001121

UniProt: Q08117

Pathways: WNT Signalweg, SARS-CoV-2 Protein Interaktom