LIG1 Antikörper (Internal Region)

Produktnummer ABIN499175

Der Ziege Polyklonal anti-LIG1 Antikörper wird verwendet zum Nachweis von LIG1 in Proben von Human und Ratte. Er wurde validiert für WB.

Kurzübersicht für LIG1 Antikörper (Internal Region) (ABIN499175)

Target: LIG1 (Ligase I, DNA, ATP-Dependent (LIG1))

Reaktivität: Human, Ratte

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser LIG1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-LIG1 Antikörper

Bindungsspezifität: Internal Region

Sequenz: C-RVREDKQPEQ ATTS

Spezifität: This antibody detects DNA ligase 1 (Internal).

Kreuzreaktivität (Details): Species reactivity (expected):RatSpecies reactivity (tested):Human

Aufreinigung: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from the internal region (near C-Terminus) of the protein sequence according to NP_000225.1

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: 1: 16000 (limit dilution). Western blot: 0.1-0.3 μg/mL. Approx 125 kDa band observed in nuclear lysates of cell lineHeLa (calculated MW of 105 kDa according to NP_000225.1). The observed molecularweight corresponds to earlier findings with different antibodies from other commercialsources.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 2 - 8 °C up to one week or (in aliquots) at -20 °C for longer.

Details zu LIG1

Target: LIG1 (Ligase I, DNA, ATP-Dependent (LIG1))

Andere Bezeichnung: DNA Ligase 1

Hintergrund: Eukaryotic DNA ligases are ATP-dependent enzymes that catalyse the joining of single and double-strand DNA breaks, which is an essential final step in DNA replication, recombination and repair. Four biochemically distinct DNA ligases, termed ligases I-IV, have been identified in mammalian cells. DNA ligase I is functionally homologous to the DNA ligase encoded by the Saccharomyces cerevisiae Cdc9 gene. The joining of Okazaki fragments during lagging strand DNA replication in mammalian cells is due to DNA ligase I.Synonyms: DNA ligase 1, DNA ligase I, EC=6.5.1.1, LIG-1, LIG1, Polydeoxyribonucleotide synthase [ATP] 1

Gen-ID: 3978

NCBI Accession: NP_000225

UniProt: P18858

Pathways: Telomere Maintenance, DNA Reparatur, DNA Replication, Synthesis of DNA