MYO5A Antikörper (Internal Region)

Produktnummer ABIN499147

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch MYO5A in WB, IF und EIA. Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte und Rind (Kuh).

Kurzübersicht für MYO5A Antikörper (Internal Region) (ABIN499147)

Target: MYO5A (Myosin VA (MYO5A))

Reaktivität: Human, Maus, Ratte, Rind (Kuh)

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser MYO5A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Produktdetails anti-MYO5A Antikörper

Bindungsspezifität: Internal Region

Sequenz: C-ETKQLELDLN

Spezifität: This antibody is expected to recognize both reported isoforms (NP_000250.3 and NP_001135967.1).

Kreuzreaktivität (Details): Species reactivity (expected):Rat, Bovine.
Species reactivity (tested):Human, Mouse.

Aufreinigung: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide sequence from the internal region of the protein sequence according to NP_000250.3, NP_001135967.1. Genename: MYO5A

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Limit Dilution: 1/8000. Western blot: 1-3 μg/mL. This antibody detects a band of approx. 200 kDa in lysates of cell lineJurkat.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu MYO5A

Target: MYO5A (Myosin VA (MYO5A))

Andere Bezeichnung: Myosin-Va

Hintergrund: Myosin-Va has been implicated in the regulation of vesicle trafficking in neurons and melanocytes. It has been suggested that class V myosins associate with membrane vesicles through their C-terminal tails. Myosin V associates with synaptic vesicles and forms a stable complex with the synaptic vesicle membrane proteins, synaptobrevin II, and synaptophysin. The dilute Myo5a gene mutation in myosin Va leads to mental retardation, variable cellular immunodeficiency and impaired melanocyte function (pigmentary dilution), known as Griscelli syndrome in humans. Myosin Va regulates melanosome distribution along microfilaments. It is found in association with the centrosome at all stages of the cell cycle. In the interphase stage, myosin Va is found in pericentriolar material. During cell division, it is found in the cytoplasm and concentrates in a trail between migrating centrioles and in the mitotic spindle poles and spindle fibers.Synonyms: Dilute myosin heavy chain, MYH12, MYO5A, Myosin heavy chain 12, Myoxin, non-muscle

Molekulargewicht: 215kDa (NP_000250.3).

Gen-ID: 4644

NCBI Accession: NP_000250

UniProt: Q9Y4I1

Pathways: Hormone Transport, Peptide Hormone Metabolism