Phospholamban Antikörper (N-Term)

Produktnummer ABIN499141

Dieser Ziege Polyklonal Antikörper detektiert spezifisch Phospholamban in WB und EIA. Es zeigt Reaktivität gegenüber Proben von Human, Ratte, Rind (Kuh) und Schwein.

Kurzübersicht für Phospholamban Antikörper (N-Term) (ABIN499141)

Target: Phospholamban (PLN)

Reaktivität: Human, Ratte, Rind (Kuh), Schwein

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Phospholamban Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-Phospholamban Antikörper

Bindungsspezifität: N-Term

Sequenz: KVQYLTRSAI RR-C

Spezifität: Recognizes Phospholamban / PLN.

Kreuzreaktivität (Details): Species reactivity (expected):Pig, Bovine.
Species reactivity (tested):Human, Rat.

Aufreinigung: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the N Terminus of the protein sequence according to NP_002658.1. Genename: PLN

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Limit Dilution: 1/8000. Western blot: 0.01-0.03 μg/mL. This antibody detects a band of approx. 13 kDa band in Human andRat Heart lysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu Phospholamban

Target: Phospholamban (PLN)

Andere Bezeichnung: Phospholamban

Hintergrund: Phospholamban (PLB/PLN) is a 52 amino acid phosphoprotein which regulates the calcium pump of cardiac sarcoplasmic reticulum (SR). PLB is an oligomer of five identical subunits each having a cytoplasmic and transmembrane domain. The cytoplasmic domain (residues 1 to 25) contains cAMP-dependent protein kinase phosphorylation sites and is highly basic and readily cleaved by proteases, whereas the transmembrane domain (residues 25 to 52) is mostly hydrophobic, protease resistant and stabilizes the pentamer. PLB is an inhibitor of cardiac muscle sarcoplasmic reticulum Ca(2+)-ATPase in the unphosphorylated state, but inhibition is relieved upon phosphorylation of the protein. The subsequent activation of the Ca(2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists. Mutations in this gene are a cause of inherited human dilated cardiomyopathy with refractory congestive heart failure.Synonyms: Cardiac phospholamban, PLB, PLN

Gen-ID: 5350

NCBI Accession: NP_002658

UniProt: P26678

Pathways: Negative Regulation of Transporter Activity