EIF3D Antikörper

Produktnummer ABIN499071

Der Kaninchen Polyklonal anti-EIF3D Antikörper (ABIN499071) detektiert spezifisch EIF3D in WB und IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human und Maus.

Kurzübersicht für EIF3D Antikörper (ABIN499071)

Target: EIF3D (Eukaryotic Translation Initiation Factor 3, Subunit D (EIF3D))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser EIF3D Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-EIF3D Antikörper

Spezifität: This antibody detects endogenous levels of eIF3ζ protein. (region surrounding Arg135)

Kreuzreaktivität (Details): Species reactivity (tested):Human, Mouse.

Aufreinigung: Affinity Chromatography using epitope-specific immunogen.

Anwendungsinformationen

Applikationshinweise: Western blot: 1/500-1/1000. Immunohistochemistry on Paraffin Sections: 1/50-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu EIF3D

Target: EIF3D (Eukaryotic Translation Initiation Factor 3, Subunit D (EIF3D))

Andere Bezeichnung: EIF3D / EIF3S7

Hintergrund: Translation initiation in eukaryotes necessitates the assembly of an 80S ribosomal complex containing methionyl initiator tRNA (Met-tRNAiMet), which is base paired at the initiation codon (AUG, GUG) in eligible transcripts. Eukaryotic initiation factors (eIFs) are utilized in a sequence of reactions that leads to 80S ribosomal assembly and initiation of translation. Eukaryotic initiation factor 3 (eIF3) is the largest family of eIFs and consists of at least 12 unique subunits in mammals. eIFE, also known as eIF p47, binds to the 40S ribosome and promotes the binding of methionyl-tRNAi and mRNA and associates with the complex p170-eIF3.Synonyms: Eukaryotic translation initiation factor 3 subunit 7, Eukaryotic translation initiation factor 3 subunit D, eIF-3-zeta, eIF3 p66

Molekulargewicht: approx. 64 kDa

Gen-ID: 8664

NCBI Accession: NP_003744

UniProt: O15371

Pathways: Ribonucleoprotein Complex Subunit Organization