AFT1 Antikörper

Produktnummer ABIN498985

Produktdetails anti-AFT1 Antikörper

Target: AFT1 (Activating Transcription Factor 1 (AFT1))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser AFT1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Spezifität: This antibody detects endogenous levels of ATF-1 protein. (region surrounding Ile57)

Kreuzreaktivität (Details): Species reactivity (expected):Mouse.
Species reactivity (tested):Human.

Aufreinigung: Affinity chromatography

Reinheit: > 95 % by SDS-PAGE

Anwendungsinformationen

Applikationshinweise: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu AFT1

Target: AFT1 (Activating Transcription Factor 1 (AFT1))

Andere Bezeichnung: ATF1 (AFT1 Produkte)

Synonyme: ews-atf1 antikoerper, fus/atf-1 antikoerper, treb36 antikoerper, ATF1 antikoerper, EWS-ATF1 antikoerper, FUS/ATF-1 antikoerper, TREB36 antikoerper, Atf-1 antikoerper, wu:fc92c07 antikoerper, zgc:64099 antikoerper, activating transcription factor 1 antikoerper, activating transcription factor 1 L homeolog antikoerper, atf1 antikoerper, ATF1 antikoerper, MGC85066 antikoerper, atf1.L antikoerper, Atf1 antikoerper

Hintergrund: Eukaryotic gene transcription is regulated by sequencespecific transcription factors that bind modular cis acting promoter and enhancer elements. The ATF/ CREB transcription factor family binds the palindromic cAMP response element (CRE) octanucleotide TGACGTCA. The ATF/CREB family includes CREB-1, CREB-2 (also designated ATF-4), ATF-1, ATF-2 and ATF-3. This family of proteins contain highly divergent N-terminal domains, but share a C-terminal leucine zipper for dimerization and DNA binding. ATF-1 is shown to play a key role in the induction of NOX1, this protein binds the cAMP response element (CRE) and mediates PKAinduced stimulation of CRE-reporter genes. ATF-2 forms homodimers and heterodimers with c-Jun to initiate CREdependent transcription. Phosphorylation of ATF-2 at Thr 69 and Thr 71 by stress-activated kinases is necessary for transcriptional activation. Myc also induces phosphorylation of ATF-2 at Thr 69 and Thr 71 to prolong the half-life of ATF-2. ATF-2 functions as a histone acetyltransferase (HAT) and acetylates Histones H2B and H4 specifically in vitro.Synonyms: ATF-1, Activating transcription factor 1, Cyclic AMP-dependent transcription factor ATF-1, Protein TREB36, cAMP-dependent transcription factor ATF-1

Molekulargewicht: approx. 29 kDa

Gen-ID: 466

NCBI Accession: NP_005162

UniProt: P18846

Pathways: Neurotrophin Signalübertragung, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Toll-Like Receptors Cascades