Leptin Receptor Antikörper

Produktnummer ABIN498483

Der Kaninchen Polyklonal anti-Leptin Receptor Antikörper (ABIN498483) detektiert spezifisch Leptin Receptor in WB und IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für Leptin Receptor Antikörper (ABIN498483)

Target: Leptin Receptor (LEPR)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Leptin Receptor Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-Leptin Receptor Antikörper

Spezifität: This antibody detects endogenous levels of Ob-R protein. (region surrounding Leu662)

Kreuzreaktivität (Details): Species reactivity (tested):Human.

Aufreinigung: Affinity chromatography

Reinheit: > 95 % by SDS-PAGE

Anwendungsinformationen

Applikationshinweise: Western Blot: 1/500. Immunohistochemistr: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu Leptin Receptor

Target: Leptin Receptor (LEPR)

Andere Bezeichnung: CD295 / Leptin Receptor

Hintergrund: Although there is substantial evidence that body weight is physiologically regulated, the molecular basis of obesity is unknown. Five single-gene mutations in mice that result in an obese phenotype have been identified. The first such recessive obesity mutation, the obese mutation (Ob), was identified in 1950. Mutation of Ob results in profound obesity and type II diabetes as part of a syndrome that resembles morbid obesity in humans. It has been postulated that the Ob gene product may function as a component of a signaling pathway in adipose tissue that functions to regulate body fat depot size. The cloning and sequence analysis of the mouse Ob gene and its human homolog has recently been described. Ob encodes an adipose tissue-specific mRNA with a highly conserved 167 amino acid open reading frame. The predicted amino acid sequence is 84 % identical between human and mouse and has the features of a secreted protein. A nonsense mutation in codon 105 has been found in the original congenic C57BL/6J Ob/Ob mouse strain. The Ob gene encodes the protein leptin.Synonyms: DB, HuB219, LEP-R, LEPR, OB receptor, OB-R, OBR

Molekulargewicht: approx. 150 kDa

Gen-ID: 3953

NCBI Accession: NP_001003679

UniProt: P48357

Pathways: JAK-STAT Signalweg, AMPK Signaling, Feeding Behaviour