SIAH2 Antikörper

Produktnummer ABIN498391

Produktdetails anti-SIAH2 Antikörper

Target: SIAH2 (Siah E3 Ubiquitin Protein Ligase 2 (SIAH2))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SIAH2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Spezifität: This antibody detects endogenous levels of Siah-2 protein. (region surrounding Glu266)

Kreuzreaktivität (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Aufreinigung: Affinity chromatography

Reinheit: > 95 % by SDS-PAGE

Anwendungsinformationen

Applikationshinweise: Western Blot: 1/500 - 1/1000. Immunhistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu SIAH2

Target: SIAH2 (Siah E3 Ubiquitin Protein Ligase 2 (SIAH2))

Andere Bezeichnung: SIAH2 (SIAH2 Produkte)

Synonyme: hSiah2 antikoerper, Xsiah-2 antikoerper, siah2-A antikoerper, xsiah2 antikoerper, AA415433 antikoerper, Sinh2 antikoerper, siah E3 ubiquitin protein ligase 2 antikoerper, siah E3 ubiquitin protein ligase 2 L homeolog antikoerper, seven in absentia 2 antikoerper, SIAH2 antikoerper, siah2.L antikoerper, Siah2 antikoerper

Hintergrund: SIAH-2 (seven in absentia homolog 2) is an E3 ligase that catalyzes ubiquitination and proteasome-mediated degradation of protein substrates. SIAH-2 encodes a 324 amino acid protein that shares 77 % identity with human SIAH-1 and 68 % identity with the Drosophila sina (seven in absentia) gene, on which development of the Drosophila R7 photoreceptor is dependent. SIAH-2 targets TRAF2 (which regulates cell responses to stress and cytokines through the regulation of key stress-signaling cascades) for degradation under stress conditions such as hypoxia. It targets HIF-1alpha prolyl hydroxylase 3 (PHD3) for degradation upon exposure to hypoxic conditions, which coincides with an increase in SIAH-2 transcription. SIAH-2 can decrease TNF-alpha-dependent induction of JNK activity and transcriptional activation of NFkappaB. SIAH-2 null mice subjected to hypoxia display an impaired respiratory response and reduced levels of hemoglobin.Synonyms: E3 ubiquitin-protein ligase SIAH2, Seven in absentia homolog 2, Siah-2

Molekulargewicht: approx. 38 kDa

Gen-ID: 6478

NCBI Accession: NP_005058

UniProt: O43255