GTF3C3 Antikörper

Produktnummer ABIN498362

Der Kaninchen Polyklonal anti-GTF3C3 Antikörper wird verwendet zum Nachweis von GTF3C3 in Proben von Human und Maus. Er wurde validiert für WB und IHC (p).

Kurzübersicht für GTF3C3 Antikörper (ABIN498362)

Target: GTF3C3 (General Transcription Factor IIIC, Polypeptide 3, 102kDa (GTF3C3))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GTF3C3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-GTF3C3 Antikörper

Spezifität: This antibody detects endogenous levels of TFIIIC102 protein. (region surrounding Glu131)

Kreuzreaktivität (Details): Species reactivity (expected):Mouse.
Species reactivity (tested):Human.

Aufreinigung: Affinity chromatography

Reinheit: > 95 % by SDS-PAGE

Anwendungsinformationen

Applikationshinweise: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu GTF3C3

Target: GTF3C3 (General Transcription Factor IIIC, Polypeptide 3, 102kDa (GTF3C3))

Andere Bezeichnung: GTF3C3

Hintergrund: RNA polymerase (pol) III synthesizes tRNA, 5s rRNA, 7SL RNA and U6 snRNA and is overexpressed in many transformed cell lines and tumors in vivo, since cells must duplicate its protein components before division. Therefore, in order to maintain rapid growth, cells must produce a high level of Pol III transcribed RNA, which requires the presence of the TFIIIB and TFIIIC2 transcription factor complexes. The TFIIIC2 complex is composed of five subunits, TFIIIC220, TFIIIC110, TFIIIC102, TFIIIC90 and TFIIIC63, that are overexpressed in adenovirus transformed cells as well as in malignant cells in vivo, such as ovarian carcinomas. TFIIIC2 recruits RNA pol III and TFIIIB to promoter elements and may be a key component in the deregulation of malignant cells. The TFIIIB complex includes the TATA-binding protein (TBP), TFIIB-related factor 1 (BRF1) and TFIIIB, the expression of which are also upregulated in transformed cells. In many carcinomas, the tumor suppressors retinoblastoma (RB) and p53 are inactivated, which affects their ability to bind and inactivate the function of TFIIIB.Synonyms: General transcription factor 3C polypeptide 3, TFIIIC 102 kDa subunit, TFIIIC gamma, TFIIIC102, Transcription factor IIIC subunit gamma

Molekulargewicht: approx. 101 kDa

Gen-ID: 9330

NCBI Accession: NP_036218

UniProt: Q9Y5Q9