NCF1 Antikörper

Produktnummer ABIN498263

Der Kaninchen Polyklonal anti-NCF1 Antikörper wird verwendet zum Nachweis von NCF1 in Proben von Human. Er wurde validiert für WB, IF und IHC (p).

Kurzübersicht für NCF1 Antikörper (ABIN498263)

Target: NCF1 (Neutrophil Cytosol Factor 1 (NCF1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NCF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-NCF1 Antikörper

Spezifität: This antibody detects endogenous levels of p47-phox protein.

Kreuzreaktivität (Details): Species reactivity (tested):Human.

Aufreinigung: Affinity chromatography using epitope-specific immunogen

Reinheit: > 95 % (by SDS-PAGE)

Anwendungsinformationen

Applikationshinweise: Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 200. Immunoflourescence: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Details zu NCF1

Target: NCF1 (Neutrophil Cytosol Factor 1 (NCF1))

Andere Bezeichnung: NCF1

Hintergrund: The heredity disease chronic granulomatous disease (CGF) has been linked to mutations in p47-phox and p67-phox. The cytosolic proteins p47-phox and p67-phox, also designated neutrophil cytosol factor (NCF)1 and NCF2, respectively, are required for activation of the superoxide-producing NADPH oxidase in neutrophils and other phagocytic cells. During activation of the NADPH oxidase, p47-phox and p67-phox migrate to the plasma membrane where they associate with cytochrome b558 and the small G protein Rac to form the functional enzyme complex. Both p47-phox and p67-phox contain two Src homology 3 (SH3) domains. The C-terminal SH3 doamin of p67-phox has been shown to interact with the proline rich domain of p47-phox, suggesting that p47-phox may faciliate the transport of p67-phox to the membrane.Synonyms: 47 kDa autosomal chronic granulomatous disease protein, 47 kDa neutrophil oxidase factor, NCF-1, NCF-47K, NOXO2, Neutrophil NADPH oxidase factor 1, Neutrophil cytosol factor 1, Nox organizer 2, Nox-organizing protein 2, SH3 and PX domain-containing protein 1A, SH3PXD1A, p47-phox

Molekulargewicht: approx. 44 kDa

Gen-ID: 653361

NCBI Accession: NP_000256

UniProt: P14598

Pathways: PI3K-Akt Signalweg