Separase Antikörper

Produktnummer ABIN498181

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch Separase in WB, IF und IHC (p). Es zeigt Reaktivität gegenüber Proben von Human und Maus.

Kurzübersicht für Separase Antikörper (ABIN498181)

Target: Separase (ESPL1) (Extra Spindle Poles Like 1 (ESPL1))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Separase Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-Separase Antikörper

Spezifität: This antibody detects endogenous levels of Separase protein.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse.
Species reactivity (tested):Human.

Aufreinigung: Affinity chromatography using epitope-specific immunogen

Reinheit: > 95 % (by SDS-PAGE)

Anwendungsinformationen

Applikationshinweise: Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 200. Immunoflourescence: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Details zu Separase

Target: Separase (ESPL1) (Extra Spindle Poles Like 1 (ESPL1))

Andere Bezeichnung: Separin / ESPL1

Hintergrund: Separase is a cysteine protease that triggers anaphase in all eukaryotes by participating in separation of sister chromatids during mitosis. Once activated, separase hydrolyzes the SCC1 subunit of cohesin, the chromosomal protein complex responsible for sister chromatid cohesion. Separase and cohesin are highly conserved from yeasts to humans. When the cell is not dividing, separase is prevented from cleaving cohesin through its association with another protein, securin. When anaphase is signaled, the securin is ubiquitinated and hydrolyzed by APC/cyclosome, releasing the active separase. Separase is transiently activated between the two meioses and may also be involved in homolog separation.Synonyms: Caspase-like protein ESPL1, ESP1, Extra spindle poles-like 1 protein, KIAA0165, Separase

Molekulargewicht: approx. 230 kDa

Gen-ID: 9700

NCBI Accession: NP_036423

UniProt: Q14674