PLCB3 Antikörper

Produktnummer ABIN498179

Der Kaninchen Polyklonal anti-PLCB3 Antikörper wird verwendet zum Nachweis von PLCB3 in Proben von Human, Ratte und Maus. Er wurde validiert für WB und IHC (p).

Kurzübersicht für PLCB3 Antikörper (ABIN498179)

Target: PLCB3 (phospholipase C, beta 3 (Phosphatidylinositol-Specific) (PLCB3))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PLCB3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-PLCB3 Antikörper

Spezifität: This antibody detects endogenous levels of PLC β3 protein.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Aufreinigung: Affinity chromatography using epitope-specific immunogen

Reinheit: > 95 % (by SDS-PAGE)

Anwendungsinformationen

Applikationshinweise: Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Details zu PLCB3

Target: PLCB3 (phospholipase C, beta 3 (Phosphatidylinositol-Specific) (PLCB3))

Andere Bezeichnung: PLCB3

Hintergrund: Phosphoinositide-specific phospholipase C (PLC) plays a critical role in the initiation of receptor mediated signal transduction through the generation of the two second messengers, inositol 1, 4, 5-triphosphate and diacylglycerol from phosphatidylinositol 4, 5 bisphosphate. A total of eight mammalian PLC isozymes have been described (PLC β1, PLC β2, PLC β3, PLC β4, PLC γ1, PLC γ2, PLC δ1 and PLC δ2) with molecular weights ranging from 85 to 150 kDa. The γ-type enzymes are unique in that they contain SH2 and SH3 domains. Moreover, the two γ-type enzymes, but not the β and δ isozymes, are subject to activation by a number of protein tyrosine kinases which associate with their SH2 domains and induce their activation by phosphoryation. In contrast, activation of PLC β1, PLC β2 and PLC β3 is mediated by the α subunits of the Gq class of heterotrimeric G proteins and by certain βγ G protein subunits. The regulatory mechanisms for PLC δ1 and PLC δ2 are as yet not resolved.Synonyms: 1-phosphatidylinositol-4, 5-bisphosphate phosphodiesterase beta-3, PLC-beta-3, Phosphoinositide phospholipase C-beta-3, Phospholipase C-beta-3

Molekulargewicht: approx. 150 kDa

Gen-ID: 5331

NCBI Accession: NP_000923

UniProt: Q01970

Pathways: WNT Signalweg, Thyroid Hormone Synthesis, Myometrial Relaxation and Contraction, CXCR4-mediated Signaling Events, G-protein mediated Events