RPA2 Antikörper (AA 115-129)

Produktnummer ABIN498167

Der Kaninchen Polyklonal anti-RPA2 Antikörper wird verwendet zum Nachweis von RPA2 in Proben von Human und Maus. Er wurde validiert für WB, IF und IHC (p).

Kurzübersicht für RPA2 Antikörper (AA 115-129) (ABIN498167)

Target: RPA2 (Replication Protein A2, 32kDa (RPA2))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RPA2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-RPA2 Antikörper

Bindungsspezifität: AA 115-129

Spezifität: This antibody detects endogenous levels of RFA2 protein.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse.
Species reactivity (tested):Human.

Aufreinigung: Affinity chromatography using epitope-specific immunogen

Reinheit: > 95 % (by SDS-PAGE)

Immunogen: synthetic peptide corresponding to aa 115-129 (of 273) of yeast DNA Replication Factor A (RFA2). The RFA complex consists of a heterotrimer of RFA1, RFA2 and RFA3, 69, 36 and 13 kda chains respectively.

Anwendungsinformationen

Applikationshinweise: Western blot: 1: 500 - 1: 1000. immunohistochemistry on paraffin sections: 1: 50 - 1: 200. Immunoflourescence: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Details zu RPA2

Target: RPA2 (Replication Protein A2, 32kDa (RPA2))

Andere Bezeichnung: RPA2

Hintergrund: This phospho specific polyclonal antibody is specific for phosphorylated pS122 of yeast RFA2. Reactivity with non-phosphorylated yeast RFA2 is minimal by ELISA. Immunogen: synthetic peptide corresponding to aa 115-129 (of 273) of yeast DNA Replication Factor A (RFA2). The RFA complex consists of a heterotrimer of RFA1, RFA2 and RFA3, 69, 36 and 13 kda chains respectively.Synonyms: REPA2, RF-A protein 2, RFA2, RP-A p32, RP-A p34, RPA32, RPA34, Replication factor A protein 2, Replication protein A, Replication protein A 32 kDa subunit, Replication protein A 34 kDa subunit

Molekulargewicht: approx. 32 kDa

Gen-ID: 855404

NCBI Accession: NP_014087

UniProt: P26754

Pathways: Telomere Maintenance, DNA Reparatur, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA