Der Kaninchen-Polyklonal Anti-RPA2-Antikörper wurde für WB, IF und IHC (p) validiert. Er ist geeignet zum Nachweis von RPA2 in Proben aus Human und Maus.
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This antibody detects endogenous levels of RFA2 protein.
Kreuzreaktivität (Details)
Species reactivity (expected):Mouse. Species reactivity (tested):Human.
Aufreinigung
Affinity chromatography using epitope-specific immunogen
Reinheit
> 95 % (by SDS-PAGE)
Immunogen
synthetic peptide corresponding to aa 115-129 (of 273) of yeast DNA Replication Factor A (RFA2). The RFA complex consists of a heterotrimer of RFA1, RFA2 and RFA3, 69, 36 and 13 kda chains respectively.
RPA2
Reaktivität: Human
WB, ELISA, IHC
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
Western blot: 1: 500 - 1: 1000. immunohistochemistry on paraffin sections: 1: 50 - 1: 200. Immunoflourescence: 1: 50 - 1: 200. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Avoid repeated freezing and thawing.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.
Target
RPA2
(Replication Protein A2, 32kDa (RPA2))
Andere Bezeichnung
RPA2
Hintergrund
This phospho specific polyclonal antibody is specific for phosphorylated pS122 of yeast RFA2. Reactivity with non-phosphorylated yeast RFA2 is minimal by ELISA. Immunogen: synthetic peptide corresponding to aa 115-129 (of 273) of yeast DNA Replication Factor A (RFA2). The RFA complex consists of a heterotrimer of RFA1, RFA2 and RFA3, 69, 36 and 13 kda chains respectively.Synonyms: REPA2, RF-A protein 2, RFA2, RP-A p32, RP-A p34, RPA32, RPA34, Replication factor A protein 2, Replication protein A, Replication protein A 32 kDa subunit, Replication protein A 34 kDa subunit