LAMP1 Antikörper

Produktnummer ABIN497981

Der Kaninchen Polyklonal anti-LAMP1 Antikörper wird verwendet zum Nachweis von LAMP1 in Proben von Human, Maus und Ratte. Er wurde validiert für WB, IF und EIA.

Kurzübersicht für LAMP1 Antikörper (ABIN497981)

Target: LAMP1 (Lysosomal-Associated Membrane Protein 1 (LAMP1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LAMP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Produktdetails anti-LAMP1 Antikörper

Spezifität: Recognizes CD107a/LAMP1 (Center).

Kreuzreaktivität (Details): Species reactivity (tested):Human, Mouse, Rat

Aufreinigung: Immunoaffinity Chromatography

Immunogen: LAMP1 antibody was raised against a 15 amino acid peptide from near the center of Human LAMP-1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA. Western blot: CD107a/LAMP1 antibody can be used for the detection of CD107a/LAMP1 at1-2 μg/mL. Despite its predicted size, LAMP-1 migrates at 110 kDa in SDS-PAGE. Positive Control: EL4 Cell Lysate. Immunofluorescence. Positive Control: Human Colon Tissue Lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS containing 0.02 % Sodium Azide as preservative

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C. Antibodies should not be exposed to prolonged high temperatures.

Details zu LAMP1

Target: LAMP1 (Lysosomal-Associated Membrane Protein 1 (LAMP1))

Andere Bezeichnung: CD107a / LAMP1

Hintergrund: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components (1,2) and is negatively regulated by TOR (Target of rapamycin) (3). A protein recently found to be involved in autophagy, LAMP-2, is a highly glycosylated protein associated with the lysosome (4,5). LAMP-1 shares much homology to LAMP-2 and is thought to have overlapping functions. Mice lacking LAMP-1 had very minor defects compared to those deficient in LAMP-2 expression (6). However, the loss of both proteins resulted in embryonic lethality (7), suggesting that each protein possesses some unique and necessary functions.Synonyms: CD107 Antigen-like Family Member A, LAMP-1, LAMPA, LGP120, Lysosome-associated membrane glycoprotein 1

Gen-ID: 3916

NCBI Accession: NP_005552

UniProt: P11279

Pathways: Autophagie