LAMP2 Antikörper (C-Term)

Produktnummer ABIN497979

Der Kaninchen Polyklonal anti-LAMP2 Antikörper (ABIN497979) detektiert spezifisch LAMP2 in WB, IF und EIA. Dieser Antikörper reagiert spezifisch mit Proben aus Human und Maus.

Kurzübersicht für LAMP2 Antikörper (C-Term) (ABIN497979)

Target: LAMP2 (Lysosomal-Associated Membrane Protein 2 (LAMP2))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LAMP2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Produktdetails anti-LAMP2 Antikörper

Bindungsspezifität: C-Term

Spezifität: Recognizes CD107b/LAMP2 (C-term).

Kreuzreaktivität (Details): Species reactivity (tested):Human, Mouse.

Aufreinigung: Immunoaffinity Chromatography

Immunogen: LAMP2 antibody was raised against a 17 amino acid peptide from near the carboxy of Human LAMP-2 (NP_054701).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA. Western blot: Beclin-1 antibody can be used for the detection of Beclin-1 at 1-2 μg/mL. Despite its predicted size, LAMP-2 migrates at 115 kDa in SDS-PAGE. Positive Control: HepG2 Cell Lysate. Immunocytochemistry. Positive Control: HepG2 Cell Lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS containing 0.02 % Sodium Azide as preservative

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu LAMP2

Target: LAMP2 (Lysosomal-Associated Membrane Protein 2 (LAMP2))

Andere Bezeichnung: CD107b / LAMP2

Hintergrund: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components (1,2) and is negatively regulated by TOR (Target of rapamycin) (3). LAMP-2, a highly glycosylated protein associated with the lysosome (4), has recently been shown to be important in autophagy as mice deficient in this protein failed to convert autophagic vacuoles into vacuoles (5) leading to impaired degradation of long-lived proteins. This correlates with the finding that human LAMP-2 deficiency causing Danon's disease is associated with the accumulation of autophagic material in striated myocytes (6). LAMP-2 exists in multiple isoforms (7).Synonyms: LAMP-2, LAMP-2C, LAMPB, Lysosome-associated membrane glycoprotein 2

Gen-ID: 3920

NCBI Accession: NP_054701

UniProt: P13473

Pathways: Autophagie