AGO1 Antikörper (N-Term) (Biotin)

Produktnummer ABIN4966092

Der Biotin-konjugierte Kaninchen Polyklonal anti-AGO1 Antikörper (ABIN4966092) detektiert spezifisch AGO1 in WB. Dieser Antikörper reagiert spezifisch mit Proben aus Arabidopsis thaliana.

Kurzübersicht für AGO1 Antikörper (N-Term) (Biotin) (ABIN4966092)

Target: AGO1 (EIF2C1) (Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1))

Reaktivität: Arabidopsis thaliana

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser AGO1 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB)

Produktdetails anti-AGO1 Antikörper (Biotin)

Bindungsspezifität: N-Term

Verwendungszweck: Anti-AGO1 | Argonaute 1 (40 μg, Biotin conjugated)

Kreuzreaktivität (Details): Arabidopsis thaliana
Not reactive in: Chlamydomonas reinhardtii

Homologie: Brassica pekinensis,Capsella rubella,Malus domestica,Pisum sativum,Ricinus communis,Vitis vinifera

Aufreinigung: Immunogen affinity purified serum

Immunogen: KLH-conugated N-terminal peptide of Arabidopsis thaliana AGO1 UniProt: O04379

Anwendungsinformationen

Applikationshinweise: 1 : 1000 (WB)

Kommentare:

AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure.The AGO1 antibody is extremely specific to AGO1 and does not cross-react with other antibodies. The evidence is 1) the peptide to which it was raised is at the very N-terminus of the protein and is not present in other AGOs 2) aAGO1 does not cross react with the AGOs which are overexpressed (AGO2, AGO3, AGO4, AGO5, AGO6, AGO9) using a western blot.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Immunogen affinity purified serum in PBS pH 7.4, conjugated to biotin.

Handhabung: A preservative may be added for long time storage

Lagerung: 4 °C

Informationen zur Lagerung: Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.

Haltbarkeit: 12-24 months

Details zu AGO1

Target: AGO1 (EIF2C1) (Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1))

Andere Bezeichnung: argonaute 1 (AGO1)

Hintergrund:

Background: AGO1 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi).This antibody is directly conjugated to Biotin.

Additional information: Antibody binds microRNA and tasiRNAs, preference for 21nt miRNAs with 5'U.TCA acetone total protein precipitation method

Molekulargewicht: 116.4 | 130 kDa

Gen-ID: 841262

UniProt: O04379

Pathways: Fc-epsilon Rezeptor Signalübertragung, Regulatorische RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Hormone Transport, Regulation of Actin Filament Polymerization, Stem Cell Maintenance, Ribonucleoprotein Complex Subunit Organization