TBP Antikörper (AA 210-239)

Produktnummer ABIN3029099

Der Kaninchen Polyklonal Anti-TBP-Antikörper wurde für WB, ELISA, IHC, IF und FACS validiert. Er ist geeignet, TBP in Proben von Human zu detektieren.

Kurzübersicht für TBP Antikörper (AA 210-239) (ABIN3029099)

Target: TBP (TATA Box Binding Protein (TBP))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TBP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Produktdetails anti-TBP Antikörper

Bindungsspezifität: AA 210-239

Kreuzreaktivität (Details): Expected species reactivity: Bovine, Chicken, Mouse, Primate, Xenopus, Zebrafish

Aufreinigung: Purified

Immunogen: A portion of amino acids 210-239 from the human protein was used as the immunogen for this TBP antibody.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: Titration of the TBP antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,Immunofluorescence: 1:10-1:50,IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot the TBP antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Details zu TBP

Target: TBP (TATA Box Binding Protein (TBP))

Andere Bezeichnung: TBP

Hintergrund: Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. A distinctive feature of TBP is a long string of glutamines in the N-terminal. This region of the protein modulates the DNA binding activity of the C terminus, and modulation of DNA binding affects the rate of transcription complex formation and initiation of transcription. Mutations that expand the number of CAG repeats encoding this polyglutamine tract, and thus increase the length of the polyglutamine string, are associated with spinocerebellar ataxia 17, a neurodegenerative disorder classified as a polyglutamine disease.

UniProt: P20226

Pathways: WNT Signalweg