YAP1 Antikörper (pSer127)

Produktnummer ABIN3029660

Der Kaninchen Polyklonal Anti-YAP1-Antikörper wurde für ELISA und DB validiert. Er ist geeignet, YAP1 in Proben von Human zu detektieren.

Kurzübersicht für YAP1 Antikörper (pSer127) (ABIN3029660)

Target: YAP1 (Yes-Associated Protein 1 (YAP1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser YAP1 Antikörper ist unkonjugiert

Applikation: ELISA, Dot Blot (DB)

Produktdetails anti-YAP1 Antikörper

Bindungsspezifität: pSer127

Kreuzreaktivität (Details): Expected species reactivity: Mouse, Rat, Chicken

Aufreinigung: Antigen affinity purified

Immunogen: This phospho-YAP antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS127 of human YAP1.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: Titration of the phospho-YAP antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Dot blot: 1:500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot the phospho-YAP antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Details zu YAP1

Target: YAP1 (Yes-Associated Protein 1 (YAP1))

Andere Bezeichnung: YAP

Hintergrund: Transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Plays a key role to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction. Isoform 2 and isoform 3 can activate the C-terminal fragment (CTF) of ERBB4 (isoform 3).

UniProt: P46937

Pathways: MAPK Signalweg, Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha